DCPS Monoclonal antibody, PBS Only

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  WB analysis using 67311-1-Ig (same clone as 67311-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67311-1-Ig (DCPS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67311-1-Ig (same clone as 67311-1-PBS)

  WB result of DCPS antibody (67311-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCPS transfected HeLa cells. This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

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  WB analysis of rat spleen using 67311-1-Ig (same clone as 67311-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67311-1-Ig (DCPS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 67311-1-Ig (same clone as 67311-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67311-1-Ig (DCPS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67311-1-Ig (same clone as 67311-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67311-1-Ig (DCPS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67311-1-Ig (same clone as 67311-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67311-1-Ig (DCPS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 67311-1-Ig (same clone as 67311-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using DCPS antibody (67311-1-Ig, Clone: 1B2E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67311-1-PBS in a different storage buffer formulation.

DCPS Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 67311-1-PBS

Clone No.1B2E4

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

DCPS, DCS 1, DCS1, decapping enzyme, scavenger, HINT 5, HINT5, HSL1, HSPC015

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Product Information

67311-1-PBS targets DCPS in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8872

  Product name: Recombinant human DCPS protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-337 aa of BC014532

  Sequence: MADAAPQLGKRKRELDVEEAHAASTEEKEAGVGNGTCAPVRLPFSGFRLQKVLRESARDKIIFLHGKVNEASEDGDGEDAVVILEKTPFQVEQVAQLLTGSPELQLQFSNDIYSTYHLFPPRQLNDVKTTVVYPATEKHLQKYLRQDLRLIRETGDDYRNITLPHLESQSLSIQWVYNILDKKAEADRIVFENPDPSDGFVLIPDLKWNQQQLDDLYLIAICHRRGIRSLRDLTPEHLPLLRNILHQGQEAILQRYRMKGDHLRVYLHYLPSYYHLHVHFTALGFEAPGSGVERAHLLAEVIENLECDPRHYQQRTLTFALRADDPLLKLLQEAQQS

• Full Name: decapping enzyme, scavenger
• Calculated Molecular Weight: 337 aa, 39 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC014532
• Gene Symbol: DCPS
• Gene ID (NCBI): 28960
• RRID: AB_2882571
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96C86
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DcpS, which is a member of the histidine triad (HIT) superfamily of pyrophosphatases, is the first HIT protein with a defined biological function and identifies the HIT motif as a new mRNA decapping domain. DcpS can encode a 38-40 kDa protein, and has the potential to form a homodimer of 80 kDa(PMID: 12198172).