DCPS Monoclonal antibody

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  WB analysis using 67311-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67311-1-Ig (DCPS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67311-1-Ig

  WB result of DCPS antibody (67311-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCPS transfected HeLa cells.

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  WB analysis of rat spleen using 67311-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67311-1-Ig (DCPS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 67311-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67311-1-Ig (DCPS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67311-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67311-1-Ig (DCPS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67311-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67311-1-Ig (DCPS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 67311-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using DCPS antibody (67311-1-Ig, Clone: 1B2E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

DCPS Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 67311-1-Ig

Clone No.1B2E4

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

DCPS, DCS 1, DCS1, decapping enzyme, scavenger, HINT 5, HINT5, HSL1, HSPC015

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Tested Applications

• Positive WB detected in: HeLa cells, rat spleen tissue, mouse brain tissue, HepG2 cells, K-562 cells, Jurkat cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67311-1-Ig targets DCPS in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8872

  Product name: Recombinant human DCPS protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-337 aa of BC014532

  Sequence: MADAAPQLGKRKRELDVEEAHAASTEEKEAGVGNGTCAPVRLPFSGFRLQKVLRESARDKIIFLHGKVNEASEDGDGEDAVVILEKTPFQVEQVAQLLTGSPELQLQFSNDIYSTYHLFPPRQLNDVKTTVVYPATEKHLQKYLRQDLRLIRETGDDYRNITLPHLESQSLSIQWVYNILDKKAEADRIVFENPDPSDGFVLIPDLKWNQQQLDDLYLIAICHRRGIRSLRDLTPEHLPLLRNILHQGQEAILQRYRMKGDHLRVYLHYLPSYYHLHVHFTALGFEAPGSGVERAHLLAEVIENLECDPRHYQQRTLTFALRADDPLLKLLQEAQQS

• Full Name: decapping enzyme, scavenger
• Calculated Molecular Weight: 337 aa, 39 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC014532
• Gene Symbol: DCPS
• Gene ID (NCBI): 28960
• RRID: AB_2882571
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96C86
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DcpS, which is a member of the histidine triad (HIT) superfamily of pyrophosphatases, is the first HIT protein with a defined biological function and identifies the HIT motif as a new mRNA decapping domain. DcpS can encode a 38-40 kDa protein, and has the potential to form a homodimer of 80 kDa(PMID: 12198172).