Tested Applications
| Positive WB detected in | HeLa cells, rat spleen tissue, mouse brain tissue, HepG2 cells, K-562 cells, Jurkat cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human liver cancer tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67311-1-Ig targets DCPS in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8872 Product name: Recombinant human DCPS protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-337 aa of BC014532 Sequence: MADAAPQLGKRKRELDVEEAHAASTEEKEAGVGNGTCAPVRLPFSGFRLQKVLRESARDKIIFLHGKVNEASEDGDGEDAVVILEKTPFQVEQVAQLLTGSPELQLQFSNDIYSTYHLFPPRQLNDVKTTVVYPATEKHLQKYLRQDLRLIRETGDDYRNITLPHLESQSLSIQWVYNILDKKAEADRIVFENPDPSDGFVLIPDLKWNQQQLDDLYLIAICHRRGIRSLRDLTPEHLPLLRNILHQGQEAILQRYRMKGDHLRVYLHYLPSYYHLHVHFTALGFEAPGSGVERAHLLAEVIENLECDPRHYQQRTLTFALRADDPLLKLLQEAQQS Predict reactive species |
| Full Name | decapping enzyme, scavenger |
| Calculated Molecular Weight | 337 aa, 39 kDa |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC014532 |
| Gene Symbol | DCPS |
| Gene ID (NCBI) | 28960 |
| RRID | AB_2882571 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96C86 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DcpS, which is a member of the histidine triad (HIT) superfamily of pyrophosphatases, is the first HIT protein with a defined biological function and identifies the HIT motif as a new mRNA decapping domain. DcpS can encode a 38-40 kDa protein, and has the potential to form a homodimer of 80 kDa(PMID: 12198172).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DCPS antibody 67311-1-Ig | Download protocol |
| IHC protocol for DCPS antibody 67311-1-Ig | Download protocol |
| WB protocol for DCPS antibody 67311-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DCPS Monoclonal antibody (1B2E4), catalog number 67311-1-Ig, has 1 total citation. It appears in World Journal of Diabetes (Impact Factor 5.1), published in November 2025. The cited study investigated N7-methylguanosine-related gene decapping scavenger enzymes as a novel biomarker regulating epithelial cell function in diabetic foot ulcers, using IF application with human and mouse samples. The research field encompasses RNA metabolism, diabetes, and wound healing.
| Species | Application | Title |
|---|---|---|
World J Diabetes N7-methylguanosine-related gene decapping scavenger enzymes as a novel biomarker regulating epithelial cell function in diabetic foot ulcers.
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