DCUN1D1 Recombinant antibody

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  WB analysis using 85849-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85849-4-RR (DCUN1D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85849-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85849-4-RR against Human DCUN1D1 were performed. The affinity constant is 23.8 pM.

DCUN1D1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85849-4-RR

Clone No.250196B5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

DCN1, DCN1 like protein 1, DCN1-like protein 1, DCNL1, DCUN 1D1

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Tested Applications

• Positive WB detected in: A549 cells, HT-29 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85849-4-RR targets DCUN1D1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DCUN1D1 fusion protein Ag1440
• Full Name: DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae)
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC009478
• Gene Symbol: DCUN1D1
• Gene ID (NCBI): 54165
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96GG9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

It has been found that DCUN1D1, also known as squamous cell carcinoma-related oncogene, expression may play a role in development of brain metastasis in patients with Non-small cell lung carcinoma (NSCLC). DCUN1D1-positive tumor cells may have the ability to disrupt the blood-brain-barrier and colonize the brain.