DDA1 Polyclonal antibody

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  WB analysis using 14995-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14995-1-AP (DDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14995-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14995-1-AP (DDA1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 14995-1-AP

  IP result of anti-DDA1 (IP:14995-1-AP, 4ug; Detection:14995-1-AP 1:500) with HepG2 cells lysate 1080 ug.

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  IHC staining of human lung cancer using 14995-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14995-1-AP (DDA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14995-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDA1 antibody (14995-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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  FC experiment of HepG2 using 14995-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug DDA1 Polyclonal antibody (14995-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

DDA1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14995-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

Placenta cross-immune reaction antigen 1, PCIA-1, PCIA1, DET1- and DDB1-associated protein 1, C19orf58

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse liver tissue, HeLa cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

14995-1-AP targets DDA1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDA1 fusion protein Ag6981
• Full Name: DET1 and DDB1 associated 1
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC000615
• Gene Symbol: DDA1
• Gene ID (NCBI): 79016
• RRID: AB_10859095
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BW61
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDA1, also known as C19orf58 and PCIA1, belongs to the DDA1 family. DDA1 is an evolutionarily conserved gene located at 19p13.11 that may be involved in the activation of nuclear factor kappaB (NFκB). DDA1 encodes an 12 kDa protein with 102 AA residues whose orthologs share 82-92% identity with Arabidopsis, invertebrates, and vertebrates. Additionally, DDA1 might be involved in tumour formation and progression (PMID: 26942699, 28211159).

Publications

Species	Application	Title
human	WB	Nature; CRL4AMBRA1 is a master regulator of D-type cyclins.; Authors - Daniele Simoneschi
human	WB	Science; Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.; Authors - Ting Han
human	WB	Nat Chem Biol; Structural basis of indisulam-mediated RBM39 recruitment to DCAF15 E3 ligase complex.; Authors - Dirksen E Bussiere ; KD Validated
mouse	IP, WB	Curr Biol; Cryptochromes-Mediated Inhibition of the CRL4Cop1-Complex Assembly Defines an Evolutionary Conserved Signaling Mechanism.; Authors - Luca Rizzini
human	IHC,IF	EMBO Mol Med; A clinically compatible drug-screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis.; Authors - Lucía Zhu
human	WB	Cell Rep; Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15.; Authors - Tabitha C Ting