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  • KD/KO Validated

DDA1 Polyclonal antibody

DDA1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14995-1-AP

Synonyms

C19orf58, DDA1, DET1 and DDB1 associated 1, PCIA 1, PCIA1



Tested Applications

Positive WB detected inHepG2 cells, mouse liver tissue, HeLa cells
Positive IP detected inHepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14995-1-AP targets DDA1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDA1 fusion protein Ag6981
Full Name DET1 and DDB1 associated 1
Calculated molecular weight 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC000615
Gene symbol DDA1
Gene ID (NCBI) 79016
RRIDAB_10859095
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDA1, also known as C19orf58 and PCIA1, belongs to the DDA1 family. DDA1 is an evolutionarily conserved gene located at 19p13.11 that may be involved in the activation of nuclear factor kappaB (NFκB). DDA1 encodes an 12 kDa protein with 102 AA residues whose orthologs share 82-92% identity with Arabidopsis, invertebrates, and vertebrates. Additionally, DDA1 might be involved in tumour formation and progression (PMID: 26942699, 28211159).

Protocols

Product Specific Protocols
WB protocol for DDA1 antibody 14995-1-APDownload protocol
IF protocol for DDA1 antibody 14995-1-APDownload protocol
IP protocol for DDA1 antibody 14995-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

CRL4AMBRA1 is a master regulator of D-type cyclins.

Authors - Daniele Simoneschi
humanWB

Science

Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.

Authors - Ting Han
humanWB

Nat Chem Biol

Structural basis of indisulam-mediated RBM39 recruitment to DCAF15 E3 ligase complex.

Authors - Dirksen E Bussiere
  • KD Validated
mouseIP, WB

Curr Biol

Cryptochromes-Mediated Inhibition of the CRL4Cop1-Complex Assembly Defines an Evolutionary Conserved Signaling Mechanism.

Authors - Luca Rizzini
humanIHC,IF

EMBO Mol Med

A clinically compatible drug-screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis.

Authors - Lucía Zhu
humanWB

Cell Rep

Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15.

Authors - Tabitha C Ting