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- KD/KO Validated
DDA1 Polyclonal antibody
DDA1 Polyclonal Antibody for IF, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14995-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, HeLa cells, mouse liver tissue |
Positive IP detected in | HepG2 cells |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 5 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
IP | See 1 publications below |
Product Information
14995-1-AP targets DDA1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DDA1 fusion protein Ag6981 |
Full Name | DET1 and DDB1 associated 1 |
Calculated molecular weight | 12 kDa |
Observed molecular weight | 12 kDa |
GenBank accession number | BC000615 |
Gene symbol | DDA1 |
Gene ID (NCBI) | 79016 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for DDA1 antibody 14995-1-AP | Download protocol |
IF protocol for DDA1 antibody 14995-1-AP | Download protocol |
IP protocol for DDA1 antibody 14995-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Science Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. | ||
Nat Chem Biol Structural basis of indisulam-mediated RBM39 recruitment to DCAF15 E3 ligase complex.
| ||
Curr Biol Cryptochromes-Mediated Inhibition of the CRL4Cop1-Complex Assembly Defines an Evolutionary Conserved Signaling Mechanism. | ||
EMBO Mol Med A clinically compatible drug-screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis. | ||