DDA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DDA1 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14995-1-AP

Synonyms

C19orf58, DDA1, DET1 and DDB1 associated 1, PCIA 1, PCIA1

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 14995-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14995-1-AP (DDA1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14995-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14995-1-AP (DDA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14995-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14995-1-AP (DDA1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 14995-1-AP

  IP Result of anti-DDA1 (IP:14995-1-AP, 4ug; Detection:14995-1-AP 1:300) with HepG2 cells lysate 1800ug.

• 

  IF Staining of HepG2 using 14995-1-AP

  Immunofluorescent analysis of HepG2 cells using 14995-1-AP (DDA1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, mouse liver tissue
• Positive IP detected in: HepG2 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

14995-1-AP targets DDA1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDA1 fusion protein Ag6981
• Full Name: DET1 and DDB1 associated 1
• Calculated molecular weight: 12 kDa
• Observed molecular weight: 12 kDa
• GenBank accession number: BC000615
• Gene symbol: DDA1
• Gene ID (NCBI): 79016
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; CRL4AMBRA1 is a master regulator of D-type cyclins.; Authors - Daniele Simoneschi
human	WB	Science; Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15.; Authors - Ting Han
human	WB	Nat Chem Biol; Structural basis of indisulam-mediated RBM39 recruitment to DCAF15 E3 ligase complex.; Authors - Dirksen E Bussiere ; KD Validated
mouse	IP, WB	Curr Biol; Cryptochromes-Mediated Inhibition of the CRL4Cop1-Complex Assembly Defines an Evolutionary Conserved Signaling Mechanism.; Authors - Luca Rizzini
human	IHC,IF	EMBO Mol Med; A clinically compatible drug-screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis.; Authors - Lucía Zhu
human	WB	Cell Rep; Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15.; Authors - Tabitha C Ting