DDAH2 Polyclonal antibody

DDAH2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14966-1-AP

Synonyms

DDAH, DDAH 2, DDAH2, DDAHII, Dimethylargininase 2, G6a, NG30, Protein G6a, S phase protein

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Validation Data Gallery

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  WB analysis using 14966-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14966-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat colon using 14966-1-AP

  rat colon tissue were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 14966-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 14966-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 14966-1-AP

  IP result of anti-DDAH2 (IP:14966-1-AP, 3ug; Detection:14966-1-AP 1:500) with COLO 320 cells lysate 2000ug.

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  IHC staining of human colon cancer using 14966-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 14966-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 14966-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 14966-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: COLO 320 cells, mouse colon tissue, rat colon tissue
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

14966-1-AP targets DDAH2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDAH2 fusion protein Ag6831
• Full Name: dimethylarginine dimethylaminohydrolase 2
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC001435
• Gene Symbol: DDAH2
• Gene ID (NCBI): 23564
• RRID: AB_2276973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.(PMID:21898353).

Publications

Species	Application	Title
rat	WB	Oxid Med Cell Longev; Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates.; Authors - Mengyang Zhang
		Clin Sci (Lond); DDAH-2 alleviates contrast medium iopromide-induced acute kidney injury through nitric oxide synthase.; Authors - Heng-Huei Lin
human	WB	FASEB J; Neuropilin-1 maintains dimethylarginine dimethylaminohydrolase 1 expression in endothelial cells, and contributes to protection from angiotensin II-induced hypertension.; Authors - Ying Wang
human	WB	iScience; Dissecting VEGF-induced acute versus chronic vascular hyperpermeability: Essential roles of dimethylarginine dimethylaminohydrolase-1.; Authors - Ying Wang
rat	WB,IHC	PLoS One; Involvement of DDAH/ADMA/NOS/cGMP and COX-2/PTGIS/cAMP Pathways in Human Tissue Kallikrein 1 Protecting Erectile Function in Aged Rats.; Authors - Kai Cui
mouse,human	WB	Nat Commun; A multicentric consortium study demonstrates that dimethylarginine dimethylaminohydrolase 2 is not a dimethylarginine dimethylaminohydrolase; Authors - Vinitha N Ragavan ; KO Validated