Home
Products
Primary Antibodies
DDAH2 Polyclonal antibody, PBS Only (14966-1-PBS)

Filter: AllWBIPIHC

WB analysis using 14966-1-AP (same clone as 14966-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.
WB analysis using 14966-1-AP (same clone as 14966-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.
WB analysis of MCF-7 using 14966-1-AP (same clone as 14966-1-PBS)
WB result of DDAH2 antibody (14966-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDAH2 transfected MCF-7 cells. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.
WB analysis using 14966-1-AP (same clone as 14966-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14966-1-AP (DDAH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.
IP experiment of COLO 320 using 14966-1-AP (same clone as 14966-1-PBS)
IP result of anti-DDAH2 (IP:14966-1-AP, 3ug; Detection:14966-1-AP 1:500) with COLO 320 cells lysate 2000ug. This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 14966-1-AP (same clone as 14966-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 14966-1-AP (same clone as 14966-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 14966-1-AP (same clone as 14966-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 14966-1-AP (same clone as 14966-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer using 14966-1-AP (DDAH2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14966-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

DDAH2 Polyclonal antibody, PBS Only

Name: DDAH2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 14966-1-PBS

DDAH2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Cat No. 14966-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

DDAH, DDAH 2, DDAH-2, DDAHII, Dimethylargininase 2

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

14966-1-PBS targets DDAH2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6831 Product name: Recombinant human DDAH2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-285 aa of BC001435 Sequence: MGTPGEGLGRCSHALIRGVPESLASGEGAGAGLPALDLAKAQREHGVLGGKLRQRLGLQLLELPPEESLPLGPLLGDTAVIQGDTALITRPWSPARRPEVDGVRKALQDLGLRIVEIGDENATLDGTDVLFTGREFFVGLSKWTNHRGAEIVADTFRDFAVSTVPVSGPSHLRGLCGMGGPRTVVAGSSDAAQKAVRAMAVLTDHPYASLTLPDDAAADCLFLRPGLPGVPPFLLHRGGGDLPNSQEALQKLSDVTLVPVSCSELEKAGAGLSSLCLVLSTRPHS Predict reactive species
Full Name	dimethylarginine dimethylaminohydrolase 2
Calculated Molecular Weight	29 kDa
Observed Molecular Weight	30 kDa
GenBank Accession Number	BC001435
Gene Symbol	DDAH2
Gene ID (NCBI)	23564
RRID	AB_2276973
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95865
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.(PMID:21898353).