DDX17 Polyclonal antibody

• Featured Product
• KD/KO Validated

DDX17 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, PLA, IP, IHC, IF, CoIP, chIP, ELISA

Conjugate

Unconjugated

Publications

13

Reviews (1)

Cat no : 19910-1-AP

Synonyms

DDX17, DEAD box protein 17, DEAD box protein p72, P72, RH70, RNA dependent helicase p72

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Validation Data Gallery

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  WB analysis using 19910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19910-1-AP (DDX17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19910-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19910-1-AP (DDX17 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19910-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19910-1-AP (DDX17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19910-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19910-1-AP (DDX17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 19910-1-AP

  IP result of anti-DDX17 (IP:19910-1-AP, 4ug; Detection:19910-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human kidney using 19910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19910-1-AP (DDX17 antibody) at dilution of 1:1000 (under 40x lens).

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  IHC staining of human kidney using 19910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19910-1-AP (DDX17 antibody) at dilution of 1:800 (under 10x lens).

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  IHC staining of human kidney using 19910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19910-1-AP (DDX17 antibody) at dilution of 1:800 (under 40x lens).

• 

  IHC staining of human kidney using 19910-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 19910-1-AP (DDX17 antibody) at dilution of 1:1000 (under 10x lens).

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  IF Staining of HeLa using 19910-1-AP

  Immunofluorescent analysis of Hela cells, using DDX17 antibody 19910-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HEK-293 using 19910-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using DDX17 antibody (19910-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HEK-293T cells, HEK-293 cells, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Hela cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 5 publications below
• CoIP: See 2 publications below
• ChIP: See 3 publications below

Product Information

19910-1-AP targets DDX17 in WB, PLA, IP, IHC, IF, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX17 fusion protein Ag13723
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 17
• Calculated Molecular Weight: 729 aa, 80 kDa
• Observed Molecular Weight: 72-80 kDa
• GenBank Accession Number: BC000595
• Gene Symbol: DDX17
• Gene ID (NCBI): 10521
• RRID: AB_10667004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX17, also named as DEAD box protein p72 and DEAD box protein p82, is a 729 amino acid protein, which belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. DDX17 is widely expressed. Levels tend to increase during colon cancer progression, from very low in benign hyperplastic polyps to very high in tubular and villous adenomas. DDX17 as an RNA helicase unwinds RNA and alters RNA structures through ATP binding and hydrolysis. DDX17 is involved in multiple cellular processes, including pre-mRNA splicing, alternative splicing, ribosomal RNA processing and miRNA processing, as well as transcription regulation. It Regulates the alternative splicing of exons exhibiting specific features. identification and characterisation of p72, a novel human nuclear DEAD box protein, which shows a striking homology to p68. a predicted molecular mass of DDX17 is 72 kDa, but endogenous DDX17 migrates aberrantly at 79 kDa on SDS-PAGE (PMID: 8871553).

Publications

Species	Application	Title
human	CoIP,chIP	Nat Commun; Intragenic recruitment of NF-κB drives splicing modifications upon activation by the oncogene Tax of HTLV-1.; Authors - Lamya Ben Ameur
human	IP	Nucleic Acids Res; The RNA helicase DDX17 controls the transcriptional activity of REST and the expression of proneural microRNAs in neuronal differentiation.; Authors - Marie-Pierre Lambert
human	WB,IP	Nucleic Acids Res; RNA helicase-dependent gene looping impacts messenger RNA processing; Authors - Sophie Terrone ; KD Validated
human	WB	PLoS Pathog; Hepatitis B virus Core protein nuclear interactome identifies SRSF10 as a host RNA-binding protein restricting HBV RNA production.; Authors - Hélène Chabrolles
human	IF	J Biol Chem; RNA Polymerase III Regulates Cytosolic RNA:DNA Hybrids and Intracellular MicroRNA Expression.; Authors - Christine Xing'er Koo
human	PLA	iScience; Interactome analysis illustrates diverse gene regulatory processes associated with LIN28A in human iPS cell-derived neural progenitor cells; Authors - Nam-Kyung Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

N (Verified Customer) (01-28-2023)

Perfect to detect double bands of DDX17.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

DDX17 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:19910-1-AP)