|Positive WB detected in||mouse testis tissue|
|Positive IP detected in||mouse testis tissue|
|Positive IHC detected in||mouse testis tissue, mouse ovary tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
51042-1-AP targets DDX4,VASA in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DDX4,VASA fusion protein Ag0447|
|Full Name||DEAD (Asp-Glu-Ala-Asp) box polypeptide 4|
|Calculated molecular weight||690aa,76 kDa; 724aa,79 kDa|
|Observed molecular weight||79 kDa|
|GenBank accession number||BC047455|
|Gene ID (NCBI)||54514|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hetty (Verified Customer) (04-10-2018)
very good antibody.