DDX4,VASA Polyclonal antibody

DDX4,VASA Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51042-1-AP

Synonyms

DDX4, DEAD box protein 4, VASA, Vasa homolog



Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

51042-1-AP targets DDX4,VASA in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX4,VASA fusion protein Ag0447
Full Name DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
Calculated molecular weight690aa,76 kDa; 724aa,79 kDa
Observed molecular weight 79 kDa
GenBank accession numberBC047455
Gene symbol DDX4
Gene ID (NCBI) 54514
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].

Protocols

Product Specific Protocols
WB protocol for DDX4,VASA antibody 51042-1-APDownload protocol
IHC protocol for DDX4,VASA antibody 51042-1-APDownload protocol
IP protocol for DDX4,VASA antibody 51042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Generation and characteristics of human Sertoli cell line immortalized by overexpression of human telomerase.

Authors - Liping Wen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Hetty (Verified Customer) (04-10-2018)

very good antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse testis lysate