CoraLite®594-conjugated DDX4,VASA Monoclonal antibody

DDX4,VASA Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

CloneNo.

1F12C3

Cat no : CL594-67147

Synonyms

DDX4, DDX4,VASA, DEAD box protein 4, VASA, Vasa homolog

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Validation Data Gallery

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  IF Staining of mouse testis using CL594-67147

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 DDX4,VASA antibody (CL594-67147, Clone: 1F12C3 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse testis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL594-67147 targets DDX4,VASA in IF applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DDX4,VASA fusion protein Ag11630
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4
• Calculated Molecular Weight: 690aa,76 kDa; 724aa,79 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC047455
• Gene Symbol: DDX4
• Gene ID (NCBI): 54514
• RRID: AB_2920091
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEAD box proteins are characterized by nine conserved sequence motifs located on two functional domains. Domain I contains six of these motifs, including the Q motif and the Walker A motif, motifs Ia and Ib, the Walker B motif, and motif III, which may act to link ATPase and helicase activities of the protein [PMID:21653890]. DDX4, a member of the DEAD box family of ATP-dependent RNA helicases, plays a central role in several aspects of germ cell development. Its function is not only required during gametogenesis in the adult but is also essential for the specification of the germ cell lineage during embryogenesis [PMID:20016130].

Publications

Species	Application	Title
mouse	IF	Biol Reprod; ARHGEF15 in Sertoli cells contributes to germ cell development and testicular immune privilege; Authors - Feng Chen