CoraLite® Plus 488-conjugated DDX41 Recombinant monoclonal antibody

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• CoraLite® Plus 488-conjugated DDX41 Recombinant monoclonal antibody (CL488-86894)

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  IF Staining of MCF-7 using CL488-86894

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 DDX41 antibody (CL488-86894, Clone: 252011G10 ) at dilution of 1:200, CL594-phalloidin (red).

DDX41 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-86894

Clone No.252011G10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

ABS, DEAD box protein 41, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX41

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-86894 targets DDX41 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag26593

  Product name: Recombinant human DDX41 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-144 aa of BC015476

  Sequence: MEESEPERKRARTDEVPAGGSRSEAEDEDDEDYVPYVPLRQRRQLLLQKLLQRRRKGAAEEEQQDSGSEPRGDEDDIPLGPQSNVSLLDQHQHLKEKAEARKESAKEKQLKEEEKILESVAEGRALMSVKEMAKGITYDDPIKT

• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 41
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC015476
• Gene Symbol: DDX41
• Gene ID (NCBI): 51428
• RRID: AB_3747863
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UJV9
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DDX41, also named as DEAD box protein abstrakt homolog, is a 622 amino acid protein, which is a probable ATP-dependent RNA helicase. DDX41 is required during post-transcriptional gene expression.and may be involved in pre-mRNA splicing.