Tested Applications
| Positive WB detected in | Calu-3 cells, LNCaP cells, HeLa cells, U2OS cells, DU 145 cells, NCCIT cells, rabbit testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68454-1-Ig targets DDX43 in WB, ELISA applications and shows reactivity with Human, Rat, Rabbit samples.
| Tested Reactivity | Human, Rat, Rabbit |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11484 Product name: Recombinant human DDX43 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 264-648 aa of BC066938 Sequence: KPTPIQSQAWPIVLQGIDLIGVAQTGTGKTLCYLMPGFIHLVLQPSLKGQRNRPGMLVLTPTRELALQVEGECCKYSYKGLRSVCVYGGGNRDEQIEELKKGVDIIIATPGRLNDLQMSNFVNLKNITYLVLDEADKMLDMGFEPQIMKILLDVRPDRQTVMTSATWPHSVHRLAQSYLKEPMIVYVGTLDLVAVSSVKQNIIVTTEEEKWSHMQTFLQSMSSTDKVIVFVSRKAVADHLSSDLILGNISVESLHGDREQRDREKALENFKTGKVRILIATDLASRGLDVHDVTHVYNFDFPRNIEEYVHRIGRTGRAGRTGVSITTLTRNDWRVASELINILERANQSIPEELVSMAERFKAHQQKREMERKMERPQGRPKKFH Predict reactive species |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 43 |
| Calculated Molecular Weight | 648 aa, 73 kDa |
| Observed Molecular Weight | 68-73 kDa |
| GenBank Accession Number | BC066938 |
| Gene Symbol | DDX43 |
| Gene ID (NCBI) | 55510 |
| RRID | AB_3085166 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NXZ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DDX43 (DEAD-box helicase 43), also known as HAGE (helicase antigen gene), is a member of the DEAD-box protein family. DDX43 is highly expressed in many tumors and is, therefore, considered a potential target for immunotherapy.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DDX43 antibody 68454-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DDX43 Monoclonal antibody (1B3B2), catalog number 68454-1-Ig, has a total of 1 citation. The citation appears in the journal Viruses (Impact Factor 3.8), published in October 2025. The study used this antibody for Western Blot in human samples and focused on comparative transcriptomics to identify DDX43 as a cellular regulator involved in suppressing HSV-2 replication, placing it within the research fields of virology and gene expression regulation.
| Species | Application | Title |
|---|---|---|
Viruses Comparative Transcriptomics Analyses Identify DDX43 as a Cellular Regulator Involved in Suppressing HSV-2 Replication.
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