DDX43 Polyclonal antibody

DDX43 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 17591-1-AP

Synonyms

Cancer/testis antigen 13, CT13, DDX43, DEAD box protein 43, DEAD box protein HAGE, HAGE, Helical antigen

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Validation Data Gallery

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  WB analysis of HeLa using 17591-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17591-1-AP (DDX43 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17591-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17591-1-AP (DDX43 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 17591-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 17591-1-AP (DDX43 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 17591-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17591-1-AP (DDX43 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 17591-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 17591-1-AP (DDX43 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 17591-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 17591-1-AP (DDX43 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 17591-1-AP

  Immunofluorescent analysis of HepG2 cells using 17591-1-AP (DDX43 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A375 cells, HepG2 cells, PC-3 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17591-1-AP targets DDX43 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX43 fusion protein Ag11484
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 43
• Calculated Molecular Weight: 648 aa, 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: BC066938
• Gene Symbol: DDX43
• Gene ID (NCBI): 55510
• RRID: AB_2090912
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		Oncol Rep; Novel cancer-testis antigen expression on glioma cell lines derived from high-grade glioma patients.; Authors - Yasuto Akiyama