DDX50 Polyclonal antibody

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• KD/KO Validated

DDX50 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 10358-1-AP

Synonyms

Nucleolar protein Gu2, Gu-beta, Gu beta, EC:3.6.4.13, DEAD box protein 50

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Validation Data Gallery

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  WB analysis of HeLa using 10358-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10358-1-AP (DDX50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10358-1-AP

  WB result of DDX50 antibody (10358-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DDX50 transfected HeLa cells.

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  WB analysis of Jurkat using 10358-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10358-1-AP (DDX50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10358-1-AP

  IP result of anti-DDX50 (IP:10358-1-AP, 4ug; Detection:10358-1-AP 1:800) with HeLa cells lysate 1200ug.

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  IF Staining of HeLa using 10358-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX50 antibody (10358-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 1 publications below

Product Information

10358-1-AP targets DDX50 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX50 fusion protein Ag0382
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 50
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC000272
• Gene Symbol: DDX50
• Gene ID (NCBI): 79009
• RRID: AB_2245834
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DEAD box polypeptide 50 (DDX50, synonyms: GU2, GUB, MGC3199, RH-II/GuB) enzyme may be involved in ribosomal RNA synthesis or processing.

Publications

Species	Application	Title
human	WB	Cell Chem Biol; Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.; Authors - Li Wang
human	WB	Arch Virol; DDX50 inhibits the replication of dengue virus 2 by upregulating IFN-β production.; Authors - Peijun Han
human	WB	Mol Cell; Nucleolus activity-dependent recruitment and biomolecular condensation by pH sensing; Authors - Fardin Aryan
human	WB,IF	Nat Chem Biol; A chemical probe to modulate human GID4 Pro/N-degron interactions; Authors - Dominic D G Owens