|Positive WB detected in||HeLa cells, A2780 cells, COLO 320 cells, HEK-293 cells, MCF-7 cells|
|Positive IHC detected in||human liver cancer tissue, human thyroid cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
20985-1-AP targets DEPDC6/Deptor in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||DEP domain containing 6|
|Calculated molecular weight||46 kDa|
|Observed molecular weight||40-45 kDa|
|GenBank accession number||NM_022783|
|Gene ID (NCBI)||64798|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
DEPDC6, also named as DEPTOR, was identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. It negative regulator of the mTORC1 and mTORC2 signaling pathways. DEPDC6 inhibits the kinase activity of both complexes. The antibody is specific to DEPDC6.
Cancer Biother Radiopharm
p65 down-regulates DEPTOR expression in response to LPS stimulation in hepatocytes.
Stem Cell Res Ther
DEPTOR regulates osteogenic differentiation via inhibiting MEG3-mediated activation of BMP4 signaling and is involved in osteoporosis.
Inhibition of neddylation regulates dendritic cell functions via Deptor accumulation driven mTOR inactivation.
TNFAIP3-DEPTOR complex regulates inflammasome secretion through autophagy in ankylosing spondylitis monocytes.
Sci Transl Med
The PTH/PTHrP-SIK3 pathway affects skeletogenesis through altered mTOR signaling.