DEPDC6/Deptor Polyclonal antibody

DEPDC6/Deptor Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20985-1-AP

Synonyms

DEP domain containing 6, DEP.6, DEPDC6, Deptor



Tested Applications

Positive WB detected inHeLa cells, A2780 cells, COLO 320 cells, HEK-293 cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

20985-1-AP targets DEPDC6/Deptor in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name DEP domain containing 6
Calculated molecular weight 46 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberNM_022783
Gene symbol DEPDC6
Gene ID (NCBI) 64798
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DEPDC6, also named as DEPTOR, was identified as a novel mTOR-interacting protein whose over-expression in certain cell types represents a new mechanism for activating PI3K/Akt signaling and promoting cell survival. It negative regulator of the mTORC1 and mTORC2 signaling pathways. DEPDC6 inhibits the kinase activity of both complexes. The antibody is specific to DEPDC6.

Protocols

Product Specific Protocols
WB protocol for DEPDC6/Deptor antibody 20985-1-APDownload protocol
IHC protocol for DEPDC6/Deptor antibody 20985-1-APDownload protocol
IF protocol for DEPDC6/Deptor antibody 20985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Cancer Biother Radiopharm

PMID: 30892073

Authors - Mi Zhang
mouseWB

Gene

p65 down-regulates DEPTOR expression in response to LPS stimulation in hepatocytes.

Authors - Xiaoling Yu
mouseIF

Stem Cell Res Ther

DEPTOR regulates osteogenic differentiation via inhibiting MEG3-mediated activation of BMP4 signaling and is involved in osteoporosis.

Authors - Si Chen
mouseWB

Oncotarget

Inhibition of neddylation regulates dendritic cell functions via Deptor accumulation driven mTOR inactivation.

Authors - Mengmeng Cheng
humanWB

Autophagy

TNFAIP3-DEPTOR complex regulates inflammasome secretion through autophagy in ankylosing spondylitis monocytes.

Authors - Yue Zhai
humanWB,IHC

Sci Transl Med

The PTH/PTHrP-SIK3 pathway affects skeletogenesis through altered mTOR signaling.

Authors - Fabiana Csukasi