DEPDC7 Polyclonal antibody

DEPDC7 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26517-1-AP

Synonyms

TR2, Protein TR2/D15, DEP domain-containing protein 7, DEP domain containing 7

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Validation Data Gallery

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  WB analysis of HepG2 using 26517-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 26517-1-AP (DEPDC7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26517-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26517-1-AP (DEPDC7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26517-1-AP targets DEPDC7 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DEPDC7 fusion protein Ag24094
• Full Name: DEP domain containing 7
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC030970
• Gene Symbol: DEPDC7
• Gene ID (NCBI): 91614
• RRID: AB_2880539
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DEPDC7 (DEP domain-containing protein 7), also known as protein TR2/D15, is a 511 amino acid protein belonging to the DEPDC7 family. DEPDC7 contains one DEP domain and is highly expressed in liver, with lower levels in kidney. DEPDC7 exists as two isoforms produced by alternative splicing events. The gene encoding DEPDC7 maps to human chromosome 11p13 and mouse chromosome 2 E2. With approximately 135 million base pairs and 1,400 genes, chromosome 11 makes up around 4% of human genomic DNA and is considered a gene and disease association dense chromosome. DEPDC7 involves the regulation of small GTPase mediated signal transduction.