DFFA,DFF45 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 85220-5-RR (same clone as 85220-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85220-5-RR (DFFA,DFF45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85220-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85220-5-RR (same clone as 85220-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DFFA,DFF45 antibody (85220-5-RR, Clone: 242915D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85220-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85220-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85220-5-RR against Human DFFA,DFF45 were performed. The affinity constant is 1.47 nM.

DFFA,DFF45 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85220-5-PBS

Clone No.242915D6

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

DFF45, DFFA, 242915D6, DFF alpha, DFF1

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Product Information

85220-5-PBS targets DFFA,DFF45 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0244

  Product name: Recombinant human DFFA,DFF45 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 9-328 aa of BC000037

  Sequence: GVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACERELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQ

• Full Name: DNA fragmentation factor, 45kDa, alpha polypeptide
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC000037
• Gene Symbol: DFFA
• Gene ID (NCBI): 1676
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00273
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.