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  • Western Blot (WB) analysis of various lysates using DFFA,DFF45 Recombinant antibody (85220-5-RR)

    WB analysis using 85220-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85220-5-RR (DFFA,DFF45 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DFFA,DFF45 Recombinant antibody (85220-5-RR)

    IF Staining of HeLa using 85220-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DFFA,DFF45 antibody (85220-5-RR, Clone: 242915D6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85220-5-RR

    Affinity and Kinetic Characterization of 85220-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85220-5-RR against Human DFFA,DFF45 were performed. The affinity constant is 1.47 nM.

DFFA,DFF45 Recombinant antibody

DFFA,DFF45 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85220-5-RR
Clone No.242915D6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Synonyms

DFF45, DFFA, 242915D6, DFF alpha, DFF1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells, K-562 cells, MCF-7 cells, C6 cells, rat brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85220-5-RR targets DFFA,DFF45 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DFFA,DFF45 fusion protein Ag0244 Predict reactive species
Full Name DNA fragmentation factor, 45kDa, alpha polypeptide
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC000037
Gene Symbol DFFA
Gene ID (NCBI) 1676
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00273
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF), heterodimer of 40-kDa (DFFB) and 45-kDa (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.

Protocols

Product Specific Protocols
WB protocol for DFFA,DFF45 antibody 85220-5-RRDownload protocol
IF protocol for DFFA,DFF45 antibody 85220-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols