Tested Applications
| Positive WB detected in | SH-SY5Y cells, A549 cells, Y79 cells, HeLa cells, Calu-1 cells, untreated, and etoposide (60uM, 5h) treated SH-SY5Y cells |
| Positive IP detected in | SH-SY5Y cells |
| Positive FC (Intra) detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13075-1-AP targets DFNA5/GSDME in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3746 Product name: Recombinant human DFNA5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 111-460 aa of BC019689 Sequence: SQSSFGTLRKQEVDLQQLIRDSAERTINLRNPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPDAAHGISSQDGPLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVLFDDELLMVLEPVCDDLVSGLSPTVAVLGELKPRQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLLRALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISL Predict reactive species |
| Full Name | deafness, autosomal dominant 5 |
| Calculated Molecular Weight | 496 aa, 55 kDa |
| Observed Molecular Weight | 55 kDa, 35 kDa, 25 kDa |
| GenBank Accession Number | BC019689 |
| Gene Symbol | DFNA5 |
| Gene ID (NCBI) | 1687 |
| RRID | AB_2093053 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60443 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for DFNA5/GSDME antibody 13075-1-AP | Download protocol |
| IHC protocol for DFNA5/GSDME antibody 13075-1-AP | Download protocol |
| IP protocol for DFNA5/GSDME antibody 13075-1-AP | Download protocol |
| WB protocol for DFNA5/GSDME antibody 13075-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
DFNA5/GSDME Polyclonal antibody (Cat# 13075-1-AP) has 110 citations in journals including Cell Research, Advanced Materials, Advanced Science, Cell Death & Differentiation, Cell Death & Disease, Science Advances, Cell Reports, and Free Radical Biology & Medicine. Research fields center on pyroptosis, cancer biology, immunotherapy, cell death mechanisms, neuroinflammation, and metabolic disorders, with WB, IHC, and IF as primary applications across human, mouse, and rat models.
| Species | Application | Title |
|---|---|---|
Cell Res Glucose starvation mimetic aldometanib removes immune barriers permitting mice with hepatocellular carcinoma to live to normal ages. | ||
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P | ||
Adv Mater Piezoelectric Ceramic Nanofiber Aerogels Direct Neutrophil Fate for Diabetic Tissue Regeneration. | ||
Adv Mater Wireless Electro-Fenton Overcomes Tumor Electric Field Treatment Resistance.
| ||
Drug Resist Updat DEAF1 confers resistance to adriamycin-induced apoptosis and pyroptosis in multiple myeloma. | ||
ACS Nano Electric-Field-Driven Ferredoxin 1-Independent Cuproptosis Induction Overcomes Therapy-Induced Resistance in Glioblastoma. |
Reviews
What customers say
Both reviewers rated the antibody 4 out of 5 stars for Western Blot at a 1:1000 dilution. One user successfully detected GSDME in splenocyte extracts, while the other confirmed it works well for detecting GSDME in cell lysates. The only noted drawback was that, when using stronger ECL, the antibody showed some cross-reactivity with a protein in the ladder at approximately 15 kDa. Overall, the antibody is regarded as reliable for GSDME detection, with minor background concerns under high-sensitivity conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
James (Verified Customer) (11-18-2022) | Used for GSDME detection in splenocyte extracts
 |
Evelina (Verified Customer) (03-14-2022) | Good antibody to detect GSDME in cell lysates. Although does detect one of the proteins within the ladder (approx.15kDa) when using stronger ECL.
 |
