DFNA5 Polyclonal antibody

• Featured Product
• KD/KO Validated

DFNA5 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

40

Reviews (2)

Cat no : 13075-1-AP

Synonyms

Gasdermin-E, Gasdermin E, DFNA5/GSDME, GSDME, Gasdermin-E, C-terminal

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Validation Data Gallery

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  WB analysis using 13075-1-AP

  Untreated, and etoposide (60uM, 5h) treated SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5/GSDME antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 13075-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5/GSDME antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 13075-1-AP

  WB result of DFNA5/ GSDME antibody (13075-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-DFNA5/ GSDME transfected A549 cells.

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  WB analysis using 13075-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5/GSDME antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 13075-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 13075-1-AP (DFNA5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 13075-1-AP

  IP result of anti-DFNA5/GSDME (IP:13075-1-AP, 4ug; Detection:13075-1-AP 1:15000) with SH-SY5Y cells lysate 1240 ug.

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  IHC staining of mouse brain using 13075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 13075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 13075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13075-1-AP (DFNA5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of SH-SY5Y using 13075-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human DFNA5/GSDME (13075-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, A549 cells, Y79 cells, HeLa cells
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: mouse brain tissue, mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 26 publications below
• IHC: See 16 publications below
• IF: See 10 publications below
• CoIP: See 1 publications below

Product Information

13075-1-AP targets DFNA5 in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DFNA5 fusion protein Ag3746
• Full Name: deafness, autosomal dominant 5
• Calculated Molecular Weight: 496 aa, 55 kDa
• Observed Molecular Weight: 55 kDa, 35 kDa, 25 kDa
• GenBank Accession Number: BC019689
• Gene Symbol: DFNA5
• Gene ID (NCBI): 1687
• RRID: AB_2093053
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DFNA5 (deafness, autosomal dominant 5), also known as GSDME or ICERE-1, is a 496 amino acid protein that is expressed in cochlea tissue, as well as in placenta, brain, heart, liver, lung and pancreas. Defects in the gene encoding DFNA5 are the cause of non-syndromic sensorineural deafness autosomal dominant type 5 (DFNA5), a form of sensorineural hearing loss that results from damage to one of various structures that receive sound information in the brain. GSDME produced two GSDME fragments with MW of 35 kDa and 25 kDa.

Publications

Species	Application	Title
human,mouse	WB,IHC,CoIP	Cell Res; Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P; Authors - Yuan-Li Ai
human	IF	Acta Pharm Sin B; A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer; Authors - Yang Liu
mouse	WB	J Control Release; Enhancing cell pyroptosis with biomimetic nanoparticles for melanoma chemo-immunotherapy; Authors - Shiquan Sun
mouse	IF	Cell Death Differ; GSDME-mediated pyroptosis promotes inflammation and fibrosis in obstructive nephropathy.; Authors - Yinshuang Li
mouse	WB,IHC	J Nanobiotechnology; Hydralazine loaded nanodroplets combined with ultrasound-targeted microbubble destruction to induce pyroptosis for tumor treatment; Authors - Shuting Huang
		Cell Death Dis; TNFSF15 inhibits progression of diabetic retinopathy by blocking pyroptosis via interacting with GSDME; Authors - Hongkun Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

James (Verified Customer) (11-18-2022)

Used for GSDME detection in splenocyte extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Spleen

DFNA5 Antibody Western Blot validation (1/1000 dilution) in Spleen (Cat no:13075-1-AP)

FH

Evelina (Verified Customer) (03-14-2022)

Good antibody to detect GSDME in cell lysates. Although does detect one of the proteins within the ladder (approx.15kDa) when using stronger ECL.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

DFNA5 Antibody Western Blot validation (1:1000 dilution) in (Cat no:13075-1-AP)

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C