DHFRL1 Polyclonal antibody, PBS Only

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• DHFRL1 Polyclonal antibody, PBS Only (27965-1-PBS)

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  WB analysis using 27965-1-AP (same clone as 27965-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27965-1-AP (DHFRL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27965-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 27965-1-AP (same clone as 27965-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27965-1-AP (DHFRL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27965-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 27965-1-AP (same clone as 27965-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27965-1-AP (DHFRL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27965-1-PBS in a different storage buffer formulation.

DHFRL1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 27965-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

27965-1-PBS targets DHFRL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10537

  Product name: Recombinant human DHFRL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-187 aa of BC063379

  Sequence: MFLLLNCIVAVSQNMGIGKNGDLPRPPLRNEFRYFQRMTTTSSVEGKQNLVIMGRKTWFSIPEKNRPLKDRINLVLSRELKEPPQGAHFLARSLDDALKLTERPELANKVDMIWIVGGSSVYKEAMNHLGHLKLFVTRIMQDFESDTFFSEIDLEKYKLLPEYPGILSDVQEGKHIKYKFEVCEKDD

• Full Name: dihydrofolate reductase-like 1
• Calculated Molecular Weight: 187 aa, 22 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC063379
• Gene Symbol: DHFRL1
• Gene ID (NCBI): 200895
• RRID: AB_3086015
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86XF0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DHFRL1, also known as DHFR2, is a key enzyme in folate metabolism. DHFRL1 is a dihydrofolate reductase, that catalyzes the NADPH-dependent reduction of dihydrofolate to the biologically active form, tetrahydrofolate (PMID: 21876184).