DHPS Monoclonal antibody

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  WB analysis using 67998-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67998-1-Ig (DHPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67998-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67998-1-Ig (DHPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67998-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67998-1-Ig (DHPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

DHPS Monoclonal Antibody for WB, ELISA

Cat No. 67998-1-Ig

Clone No.1E4F5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

1E4F5, DHS, DS, EC:2.5.1.46, MIG13

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Tested Applications

• Positive WB detected in: HEK-293 cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67998-1-Ig targets DHPS in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag31046

  Product name: Recombinant human DHPS protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-369 aa of BC014016

  Sequence: MEGSLEREAPAGALAAVLKHSSTLPPESTQVRGYDFNRGVNYRALLEAFGTTGFQATNFGRAVQQVNAMIEKKLEPLSQDEDQHADLTQSRRPLTSCTIFLGYTSNLISSGIRETIRYLVQHNMVDVLVTTAGGVEEDLIKCLAPTYLGEFSLRGKELRENGINRIGNLLVPNENYCKFEDWLMPILDQMVMEQNTEGVKWTPSKMIARLGKEINNPESVYYWAQKNHIPVFSPALTDGSLGDMIFFHSYKNPGLVLDIVEDLRLINTQAIFAKCTGMIILGGGVVKHHIANANLMRNGADYAVYINTAQEFDGSDSGARPDEAVSWGKIRVDAQPVKVYADASLVFPLLVAETFAQKMDAFMHEKNED

• Full Name: deoxyhypusine synthase
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC014016
• Gene Symbol: DHPS
• Gene ID (NCBI): 1725
• RRID: AB_2918746
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P49366
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DHPS(Deoxyhypusine synthase) catalyzes the conversion of lysine to deoxyhypusine residue on the eukaryotic initiation factor 5A (eIF-5A) precursor using spermidine as the substrate. It belongs to the deoxyhypusine synthase family. This protein has 3 isoforms produced by alternative splicing.