DHPS Monoclonal antibody, PBS Only

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  WB analysis using 67998-1-Ig (same clone as 67998-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67998-1-Ig (DHPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67998-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 67998-1-Ig (same clone as 67998-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67998-1-Ig (DHPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67998-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67998-1-Ig (same clone as 67998-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67998-1-Ig (DHPS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67998-1-PBS in a different storage buffer formulation.

DHPS Monoclonal Antibody for WB, Indirect ELISA

Cat No. 67998-1-PBS

Clone No.1E4F5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

1E4F5, DHS, DS, EC:2.5.1.46, MIG13

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Product Information

67998-1-PBS targets DHPS in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag31046

  Product name: Recombinant human DHPS protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-369 aa of BC014016

  Sequence: MEGSLEREAPAGALAAVLKHSSTLPPESTQVRGYDFNRGVNYRALLEAFGTTGFQATNFGRAVQQVNAMIEKKLEPLSQDEDQHADLTQSRRPLTSCTIFLGYTSNLISSGIRETIRYLVQHNMVDVLVTTAGGVEEDLIKCLAPTYLGEFSLRGKELRENGINRIGNLLVPNENYCKFEDWLMPILDQMVMEQNTEGVKWTPSKMIARLGKEINNPESVYYWAQKNHIPVFSPALTDGSLGDMIFFHSYKNPGLVLDIVEDLRLINTQAIFAKCTGMIILGGGVVKHHIANANLMRNGADYAVYINTAQEFDGSDSGARPDEAVSWGKIRVDAQPVKVYADASLVFPLLVAETFAQKMDAFMHEKNED

• Full Name: deoxyhypusine synthase
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC014016
• Gene Symbol: DHPS
• Gene ID (NCBI): 1725
• RRID: AB_2918746
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P49366
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DHPS(Deoxyhypusine synthase) catalyzes the conversion of lysine to deoxyhypusine residue on the eukaryotic initiation factor 5A (eIF-5A) precursor using spermidine as the substrate. It belongs to the deoxyhypusine synthase family. This protein has 3 isoforms produced by alternative splicing.