DHX38 Polyclonal antibody, PBS Only

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• DHX38 Polyclonal antibody, PBS Only (10098-2-PBS)

• 

  WB analysis using 10098-2-AP (same clone as 10098-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10098-2-AP (DHX38 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10098-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10098-2-AP (same clone as 10098-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10098-2-AP (DHX38 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10098-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 10098-2-AP (same clone as 10098-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10098-2-AP (DHX38 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10098-2-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 10098-2-AP (same clone as 10098-2-PBS)

  IP result of anti-DHX38 (IP:10098-2-AP, 4ug; Detection:10098-2-AP 1:700) with HeLa cells lysate 1400ug. This data was developed using the same antibody clone with 10098-2-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

DHX38 Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 10098-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

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Product Information

10098-2-PBS targets DHX38 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0144

  Product name: Recombinant human DHX38 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 19-280 aa of BC004235

  Sequence: DCQVGGLICKSKSAASEQHVFKAPAPRPSLLGLDLLASLKRREREEKDDGEDKKKSKVSSYKDWEESKDDQKDAEEEGGDQAGQNIRKDRHYRSARVETPSHPGGVSEEFWERSRQRERERREHGVYASSKEEKDWKKEKSRDRDYDRKRDRDERDRSRHSSRSERDGGSERSSRRNEPESPRHRPKDAATPSRSTWEEEDSGYGSSRRSQWESPSPTPSYRDSERSHRLSTRDRDRSVRGKYSDDTPLPTPSYKYNEWADD

• Full Name: DEAH (Asp-Glu-Ala-His) box polypeptide 38
• Calculated Molecular Weight: 141 kDa
• Observed Molecular Weight: 141 kDa
• GenBank Accession Number: BC004235
• Gene Symbol: DHX38
• Gene ID (NCBI): 9785
• RRID: AB_2092294
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92620
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The protein encoded by this gene is a member of the DEAD/H box family of splicing factors, which are involved in pre-mRNA splicing. The yeast prp16 is an essential splicing factor that catalyzes RNA-dependent hydrolysis of nucleoside triphosphates, acting as an ATPase. This protein resembles yeast Prp16 more closely than other DEAD/H family members and also is a general splicing factor essential for catalytic step II during pre-mRNA processing. Western blot analysis showed it is a 140-KD nuclear protein expressed in various tissues.