DIMT1L Polyclonal antibody

DIMT1L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15563-1-AP

Synonyms

18S rRNA dimethylase, DIM1, DIMT1, DIMT1L, HSA9761, HUSSY5

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Validation Data Gallery

• 

  WB analysis using 15563-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15563-1-AP (DIMT1L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15563-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15563-1-AP (DIMT1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15563-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15563-1-AP (DIMT1L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 15563-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15563-1-AP (DIMT1L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, Raji cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15563-1-AP targets DIMT1L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DIMT1L fusion protein Ag7721
• Full Name: DIM1 dimethyladenosine transferase 1-like (S. cerevisiae)
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC002841
• Gene Symbol: DIMT1L
• Gene ID (NCBI): 27292
• RRID: AB_2090821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs; Authors - James C Murphy