DIRAS2 Polyclonal antibody

• Featured Product
• KD/KO Validated

DIRAS2 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15557-1-AP

Synonyms

GTP-binding protein Di-Ras2, GTP binding protein Di Ras2

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Validation Data Gallery

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  WB analysis of human brain using 15557-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 15557-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15557-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15557-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 15557-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15557-1-AP (DIRAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15557-1-AP

  IP result of anti-DIRAS2 (IP:15557-1-AP, 4ug; Detection:15557-1-AP 1:1000) with mouse brain tissue lysate 4400ug.

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  IF Staining of HeLa using 15557-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15557-1-AP (DIRAS2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 15557-1-AP

  Immunofluorescent analysis of Hela cells, using DIRAS2 antibody 15557-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HeLa using 15557-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug DIRAS2 Polyclonal antibody (15557-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: human brain tissue, rat brain tissue, HeLa cells, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

15557-1-AP targets DIRAS2 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DIRAS2 fusion protein Ag7926
• Full Name: DIRAS family, GTP-binding RAS-like 2
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC008065
• Gene Symbol: DIRAS2
• Gene ID (NCBI): 54769
• RRID: AB_2261563
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IP	Oncogene; Di-Ras2 promotes renal cell carcinoma formation by activating the mitogen-activated protein kinase pathway in the absence of von Hippel-Lindau protein.; Authors - Hanyu Rao ; KD Validated
human	WB	Cancers (Basel); Frequent Epigenetic Inactivation of DIRAS-1 and DIRAS-2 Contributes to Chemo-Resistance in Gliomas.; Authors - Tanja Rothhammer-Hampl
human,mouse	WB,IF	J Neural Transm (Vienna); Expression of the ADHD candidate gene Diras2 in the brain.; Authors - Lena Grünewald
human	WB	Dev Cell; The UBE2F-CRL5ASB11-DIRAS2 axis is an oncogene and tumor suppressor cascade in pancreatic cancer cells; Authors - Yu Chang