CoraLite® Plus 488-conjugated DKK3 Monoclonal antibody

DKK3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4E6H6

Cat no : CL488-66758

Synonyms

Dickkopf 3, Dickkopf related protein 3, Dkk 3, DKK3, hDkk 3, REIC, RIG



Tested Applications

Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66758 targets DKK3 in IF applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DKK3 fusion protein Ag23615
Full Name dickkopf homolog 3 (Xenopus laevis)
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 40 kDa, 50-55 kDa
GenBank Accession NumberBC007660
Gene Symbol DKK3
Gene ID (NCBI) 27122
RRIDAB_2919363
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DKK3, also named as REIC, belongs to the dickkopf family. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer's disea. The adenovirus carrying REIC/Dkk-3 (Ad-REIC) exhibits a potent tumor-specific cell-killing function for various human cancers. It has also become evident that some human cancers are resistant to Ad-REIC-induced apoptosis. The DKK3 is a Glycosylation protein with MW about 55 kDa or 38-43 kDa.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 DKK3 antibody CL488-66758Download protocol
Standard Protocols
Click here to view our Standard Protocols