DLEC1 Polyclonal antibody

DLEC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20027-1-AP

Synonyms

DLC 1, DLC1, DLEC1, F56



Tested Applications

Positive WB detected inhuman testis tissue, A549 cells, PC-3 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

20027-1-AP targets DLEC1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name deleted in lung and esophageal cancer 1
Calculated molecular weight 196 kDa
Observed molecular weight 80-90 kDa, 46 kDa
GenBank accession numberNM_007335
Gene symbol DLEC1
Gene ID (NCBI) 9940
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DLEC1, also named as DLC1 F56, may act as a tumor suppressor by inhibiting cell proliferation.

Protocols

Product Specific Protocols
WB protocol for DLEC1 antibody 20027-1-APDownload protocol
IHC protocol for DLEC1 antibody 20027-1-APDownload protocol
IF protocol for DLEC1 antibody 20027-1-APDownload protocol
IP protocol for DLEC1 antibody 20027-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols