DLEC1 Polyclonal antibody

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• DLEC1 Polyclonal antibody (20027-1-AP)

DLEC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20027-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

DLC1, F56, DLC-1, DLC 1

• 

  WB analysis of human testis using 20027-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 20027-1-AP (DLEC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 20027-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20027-1-AP (DLEC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-3 using 20027-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20027-1-AP (DLEC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of PC-3 using 20027-1-AP

  IP result of anti-DLEC1 (IP:20027-1-AP, 4ug; Detection:20027-1-AP 1:300) with PC-3 cells lysate 1100ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 20027-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20027-1-AP (DLEC1 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 20027-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20027-1-AP (DLEC1 antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of PC-3 using 20027-1-AP

  Immunofluorescent analysis of PC-3 cells using 20027-1-AP (DLEC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human testis tissue, A549 cells, PC-3 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20027-1-AP targets DLEC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: deleted in lung and esophageal cancer 1
• Calculated Molecular Weight: 196 kDa
• Observed Molecular Weight: 80-90 kDa, 46 kDa
• GenBank Accession Number: NM_007335
• Gene Symbol: DLEC1
• Gene ID (NCBI): 9940
• RRID: AB_10664924
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y238
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DLEC1, also named as DLC1 F56, may act as a tumor suppressor by inhibiting cell proliferation.

Publications

What published studies show

The DLEC1 Polyclonal antibody (Cat# 20027-1-AP) has 1 total citation. It appears in Human Genetics, a study investigating epigenetic silencing of DLEC1 in relation to the tumor immune microenvironment and immunotherapy prognosis across multiple cancers. The research field encompasses cancer epigenetics, tumor immunology, and predictive biomarkers for immunotherapy response.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Hum Genet; Epigenetic silencing of DLEC1 correlates with tumor immune microenvironment and predicts immunotherapy prognosis in multiple cancers.; Authors - Ruijie Ming