DLEC1 Polyclonal antibody

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• DLEC1 Polyclonal antibody (20027-1-AP)

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  WB analysis of human testis using 20027-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 20027-1-AP (DLEC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 20027-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20027-1-AP (DLEC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 20027-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20027-1-AP (DLEC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 20027-1-AP

  IP result of anti-DLEC1 (IP:20027-1-AP, 4ug; Detection:20027-1-AP 1:300) with PC-3 cells lysate 1100ug.

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  IHC staining of human testis using 20027-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20027-1-AP (DLEC1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 20027-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20027-1-AP (DLEC1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of PC-3 using 20027-1-AP

  Immunofluorescent analysis of PC-3 cells using 20027-1-AP (DLEC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

DLEC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 20027-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

DLC1, F56, DLC-1, DLC 1

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Tested Applications

• Positive WB detected in: human testis tissue, A549 cells, PC-3 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

20027-1-AP targets DLEC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: deleted in lung and esophageal cancer 1
• Calculated Molecular Weight: 196 kDa
• Observed Molecular Weight: 80-90 kDa, 46 kDa
• GenBank Accession Number: NM_007335
• Gene Symbol: DLEC1
• Gene ID (NCBI): 9940
• RRID: AB_10664924
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y238
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DLEC1, also named as DLC1 F56, may act as a tumor suppressor by inhibiting cell proliferation.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Hum Genet; Epigenetic silencing of DLEC1 correlates with tumor immune microenvironment and predicts immunotherapy prognosis in multiple cancers.; Authors - Ruijie Ming