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DLL4 Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 21584-1-AP

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Con: 33 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, ELISA
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Images See all

DLL4 Antibody WB mouse brain tissue 21584-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 21584-1-AP(DLL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

DLL4 Antibody IF human stomach cancer tissue 21584-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed human stomach cancer tissue using 21584-1-AP (DLL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

DLL4 Antibody WB Transfected HEK-293 cells 21584-1-AP

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 21584-1-AP( DLL4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

DLL4 Antibody IP mouse lung tissue 21584-1-AP

IP result of anti-DLL4(IP:21584-1-AP, 4ug; Detection:21584-1-AP 1:300) with mouse lung tissue lysate 4000 ug

Species specificity:
human, mouse
Positive WB detected in:
mouse brain tissue, Transfected HEK-293 cells
Positive IP detected in:
mouse lung tissue,
Positive IF detected in:
human stomach cancer tissue,
Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
DLL4 fusion protein Ag16140
Full name:
delta-like 4 (Drosophila)
Calculated molecular weight:
685aa,75 kDa
Observed molecular weight:
75-80 kDa
GenBank accession number:
BC106950
Gene ID (NCBI):
54567
Gene symbol
DLL4
Synonyms
delta like 4 (Drosophila), Delta like protein 4, Delta4, DLL4, Drosophila Delta homolog 4, hdelta2

Background

DLL4, also named as Delta4, plays a role in the Notch signaling pathway. It activates Notch-1 and Notch-4. Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. DLL4 is highly expressed in the vascular endothelium.


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