|Positive WB detected in||mouse brain tissue, A431 cells|
|Positive IP detected in||mouse lung tissue|
|Positive IF detected in||human stomach cancer tissue|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 21584-1-AP is DLL4 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DLL4 fusion protein Ag16140|
|Full Name||delta-like 4 (Drosophila)|
|Calculated molecular weight||685 aa, 75 kDa|
|Observed molecular weight||75-80 kDa|
|GenBank accession number||BC106950|
|Gene ID (NCBI)||54567|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
DLL4, also named as Delta4, plays a role in the Notch signaling pathway. It activates Notch-1 and Notch-4. Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. DLL4 is highly expressed in the vascular endothelium.
Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.
J Cell Mol Med
Long non-coding RNA GAPLINC promotes angiogenesis by regulating miR-211 under hypoxia in human umbilical vein endothelial cells.
Exp Lung Res
Role of the Notch ligands Jagged1 and Delta4 in Th17/Treg immune imbalance in a mouse model of chronic asthma.
Anti-DLL4 ameliorates toluene diisocyanate-induced experimental asthma by inhibiting Th17 response.
Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes.