DLL4 Polyclonal antibody

DLL4 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 21584-1-AP

Synonyms

delta like 4 (Drosophila), Delta like protein 4, Delta4, DLL4, Drosophila Delta homolog 4, hdelta2

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Validation Data Gallery

• 

  WB analysis of mouse brain using 21584-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21584-1-AP (DLL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of human stomach cancer using 21584-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human stomach cancer tissue using 21584-1-AP (DLL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IP experiment of mouse lung using 21584-1-AP

  IP result of anti-DLL4 (IP:21584-1-AP, 4ug; Detection:21584-1-AP 1:300) with mouse lung tissue lysate 4000 ug.

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Tested Applications

• Positive WB detected in: mouse brain tissue, A431 cells
• Positive IP detected in: mouse lung tissue
• Positive IF detected in: human stomach cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

The immunogen of 21584-1-AP is DLL4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DLL4 fusion protein Ag16140
• Full Name: delta-like 4 (Drosophila)
• Calculated molecular weight: 685 aa, 75 kDa
• Observed molecular weight: 75-80 kDa
• GenBank accession number: BC106950
• Gene symbol: DLL4
• Gene ID (NCBI): 54567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DLL4, also named as Delta4, plays a role in the Notch signaling pathway. It activates Notch-1 and Notch-4. Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. DLL4 is highly expressed in the vascular endothelium.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
human	WB	J Cell Mol Med; Long non-coding RNA GAPLINC promotes angiogenesis by regulating miR-211 under hypoxia in human umbilical vein endothelial cells.; Authors - Yangyan He
mouse	WB,IF	Angiogenesis; Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes.; Authors - Jing Bai

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