DLL4 Polyclonal antibody

DLL4 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 21584-1-AP

Synonyms

delta like 4 (Drosophila), Delta like protein 4, Delta4, DLL4, Drosophila Delta homolog 4, hdelta2



Tested Applications

Positive WB detected inmouse brain tissue, A431 cells
Positive IP detected inmouse lung tissue
Positive IF detected inhuman stomach cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 21584-1-AP is DLL4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DLL4 fusion protein Ag16140
Full Name delta-like 4 (Drosophila)
Calculated molecular weight 685 aa, 75 kDa
Observed molecular weight 75-80 kDa
GenBank accession numberBC106950
Gene symbol DLL4
Gene ID (NCBI) 54567
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DLL4, also named as Delta4, plays a role in the Notch signaling pathway. It activates Notch-1 and Notch-4. Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. DLL4 is highly expressed in the vascular endothelium.

Protocols

Product Specific Protocols
WB protocol for DLL4 antibody 21584-1-APDownload protocol
IF protocol for DLL4 antibody 21584-1-APDownload protocol
IP protocol for DLL4 antibody 21584-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Authors - Xiuying Zhong
humanWB

J Cell Mol Med

Long non-coding RNA GAPLINC promotes angiogenesis by regulating miR-211 under hypoxia in human umbilical vein endothelial cells.

Authors - Yangyan He