DLL4 Polyclonal antibody

• Featured Product
• KD/KO Validated

DLL4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 21584-1-AP

Synonyms

delta like 4 (Drosophila), Delta like protein 4, Delta4, DLL4, Drosophila Delta homolog 4, hdelta2

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Validation Data Gallery

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  WB analysis using 21584-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21584-1-AP (DLL4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21584-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21584-1-AP (DLL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 21584-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21584-1-AP (DLL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 21584-1-AP

  IP result of anti-DLL4 (IP:21584-1-AP, 4ug; Detection:21584-1-AP 1:300) with mouse lung tissue lysate 4000 ug.

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  IHC staining of mouse lung using 21584-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21584-1-AP (DLL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 21584-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21584-1-AP (DLL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human stomach cancer using 21584-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human stomach cancer tissue using 21584-1-AP (DLL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HUVEC cells, mouse brain tissue, A431 cells, bEnd.3 cells, mouse lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human stomach cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 13 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

21584-1-AP targets DLL4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DLL4 fusion protein Ag16140
• Full Name: delta-like 4 (Drosophila)
• Calculated Molecular Weight: 685 aa, 75 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC106950
• Gene Symbol: DLL4
• Gene ID (NCBI): 54567
• RRID: AB_2878888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DLL4, also named as Delta4, plays a role in the Notch signaling pathway. It activates Notch-1 and Notch-4. Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. DLL4 is highly expressed in the vascular endothelium and is involved in angiogenesis.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
human	IHC	Cell Rep Med; Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application; Authors - Xiaoxue Ren
mouse	WB,IF	Angiogenesis; Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes.; Authors - Jing Bai
human	WB	Bioact Mater; Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid.; Authors - Wenjing Liu
mouse	WB	Theranostics; Endothelial PERK-ATF4-JAG1 axis activated by T-ALL remodels bone marrow vascular niche.; Authors - Cui Liu
human	WB	EMBO Rep; Dysregulation of the miR-30c/DLL4 axis by circHIPK3 is essential for KSHV lytic replication.; Authors - Katherine L Harper ; KO Validated

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