DMC1 Monoclonal antibody

DMC1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2D5C9

Cat no : 67176-1-Ig

Synonyms

dJ199H16.1, DMC1, DMC1H, HsLim15, LIM15



Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, human testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

67176-1-Ig targets DMC1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DMC1 fusion protein Ag6951
Full Name DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast)
Calculated molecular weight 165 aa, 18 kDa, 38 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC035658
Gene symbol DMC1
Gene ID (NCBI) 11144
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DMC1 antibody 67176-1-IgDownload protocol
IHC protocol for DMC1 antibody 67176-1-IgDownload protocol
IF protocol for DMC1 antibody 67176-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Genet Genomics

The novel male meiosis recombination regulator coordinates the progression of meiosis prophase I.

Authors - Miao Li