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DMRT1 Polyclonal antibody, PBS Only (14313-1-PBS)

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WB analysis using 14313-1-AP (same clone as 14313-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14313-1-AP (DMRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14313-1-PBS in a different storage buffer formulation.
IP experiment of mouse testis using 14313-1-AP (same clone as 14313-1-PBS)
IP result of anti-DMRT1 (IP:14313-1-AP, 4ug; Detection:14313-1-AP 1:500) with mouse testis tissue lysate 2400 ug. This data was developed using the same antibody clone with 14313-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 14313-1-AP (same clone as 14313-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14313-1-AP (DMRT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14313-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 14313-1-AP (same clone as 14313-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14313-1-AP (DMRT1 Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 14313-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 14313-1-AP (same clone as 14313-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14313-1-AP (DMRT1 Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 14313-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 14313-1-AP (same clone as 14313-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14313-1-AP (DMRT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14313-1-PBS in a different storage buffer formulation.
IF Staining of mouse testis using 14313-1-AP (same clone as 14313-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DMRT1 antibody (14313-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14313-1-PBS in a different storage buffer formulation.
IF Staining of mouse testis using 14313-1-AP (same clone as 14313-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DMRT1 antibody (14313-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14313-1-PBS in a different storage buffer formulation.

DMRT1 Polyclonal antibody, PBS Only

Name: DMRT1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 14313-1-PBS

DMRT1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Cat No. 14313-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

Doublesex- and mab-3-related transcription factor 1, DMT1, CT154

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

14313-1-PBS targets DMRT1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5547 Product name: Recombinant human DMRT1 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-373 aa of BC040847 Sequence: MPNDEAFSKPSTPSEAPHAPGVPPQGRAGGFGKASGALVGAASGSSAGGSSRGGGSGSGASDLGAGSKKSPRLPKCARCRNHGYASPLKGHKRFCMWRDCQCKKCNLIAERQRVMAAQVALRRQQAQEEELGISHPIPLPSAAELLVKRENNGSNPCLMTECSGTSQPPPASVPTTAASEGRMVIQDIPAVTSRGHVENTPDLVSDSTYYSSFYQPSLFPYYNNLYNCPQYSMALAADSASGEVGNPLGGSPVKNSLRGLPGPYVPGQTGNQWQMKNMENRHAMSSQYRMHSYYPPPSYLGQSVPQFFTFEDAPSYPEARASVFSPPSSQDSGLVSLSSSSPISNKSTKAVLECEPASEPSSFTVTPVIEEDE Predict reactive species
Full Name	doublesex and mab-3 related transcription factor 1
Calculated Molecular Weight	39 kDa
Observed Molecular Weight	39 kDa
GenBank Accession Number	BC040847
Gene Symbol	DMRT1
Gene ID (NCBI)	1761
RRID	AB_2918035
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y5R6
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

DMRT1, also named DMT1, belongs to the DMRT family. DMRT1 is one of the most conserved transcription factors in a variety of species and is involved in sex determination and germ cell differentiation. DMRT1 prevents the sexual switch from male to female, represses pluripotency, and balances mitosis versus meiosis. DMRT1 also maintains spermatogenesis and replenishes mGSCs by collaborating with Plzf and Sal-like protein 4 (PMID: 33420764). DMRT1 has 3 isoforms with the molecular mass of 18, 29, and 39 kDa.