DMXL2 Monoclonal antibody

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  WB analysis of mouse cerebellum using 66891-2-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66891-2-Ig (DMXL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66891-2-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66891-2-Ig (DMXL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66891-2-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66891-2-Ig (DMXL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 66891-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DMXL2 antibody (66891-2-Ig, Clone: 1F9A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

DMXL2 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 66891-2-Ig

Clone No.1F9A8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Synonyms

Rabconnectin 3, KIAA0856, DmX-like protein 2, DmX like protein 2, Dmx like 2

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, Jurkat cells, rat brain tissue
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66891-2-Ig targets DMXL2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19861

  Product name: Recombinant human DMXL2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 573-922 aa of BC140781

  Sequence: DSHHTLLHQEGMSVGSPHGSQPHSRSHSTHMNILAPTVMMISKHIDGSLNQWAVTFADKSAFTTVLTVSHKFRYCGHRFHLNDLACHSVLPLLLTSSHHNALLTPELDCQWDSDNKLSRLMDPVKHIKGSSKQPLRNAATRTFHDPNAIYSELILWRVDPIGPLSYTGGVSELARINSLHTSAFSNVAWLPTLIPSYCLGTYCNSASACFVASDGKNLRLYQAVVDARKLLDELSDPESSKLIGEVFNIVSQQSTARPGCIIELDAITNQCGSNTQLLHVFQEDFIIGYKPHKEDMEKKETEIFFQPSQGYRPPPFSEKFFLVVIEKDSNNNSILHMWHLHLKSVQACLG

• Full Name: Dmx-like 2
• Calculated Molecular Weight: 3036 aa, 340 kDa
• Observed Molecular Weight: 340 kDa
• GenBank Accession Number: BC140781
• Gene Symbol: DMXL2
• Gene ID (NCBI): 23312
• RRID: AB_2882221
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TDJ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DMXL2, also named as rabconnectin-3, is a functional regulator of mammalian notch signaling with WD domains. Rabconnectin-3 is abundantly expressed in the brain where it is enriched in the synaptic vesicle fraction. Immunofluorescence and immunoelectron microscopy revealed that rabconnectin-3 was concentrated on synaptic vesicles at synapses. It may serve as a scaffold molecule for both Rab3 GEP and GAP on synaptic vesicles.