Tested Applications
| Positive WB detected in | mouse liver tissue, mouse thymus tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18727-1-AP targets DNA2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | DNA replication helicase 2 homolog (yeast) |
| Calculated Molecular Weight | 120 kDa |
| Observed Molecular Weight | 120-130 kDa |
| GenBank Accession Number | NM_001080449 |
| Gene Symbol | DNA2 |
| Gene ID (NCBI) | 1763 |
| RRID | AB_10637998 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51530 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNA2(DNA replication ATP-dependent helicase/nuclease DNA2) is also named as DNA2L, KIAA0083 and belongs to the DNA2/NAM7 helicase family. This protein includes 2 domains: DNA replication nuclease and DNA replication ATP-dependent helicase. It is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability and it can interact with DNA polymerase gamma and stimulating its activity. It can also process intermediate 5' flap structures occuring in DNA replication long-patch base excision repair in mitochondria. It has 4 isoforms produced by alternative splicing. This antibody is specific to DNA2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DNA2 antibody 18727-1-AP | Download protocol |
| IHC protocol for DNA2 antibody 18727-1-AP | Download protocol |
| IP protocol for DNA2 antibody 18727-1-AP | Download protocol |
| WB protocol for DNA2 antibody 18727-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DNA2 Polyclonal antibody (Cat# 18727-1-AP) has 20 citations spanning journals including Nature Cell Biology, Molecular Cell, Nucleic Acids Research, Cell Reports Medicine, EMBO Molecular Medicine, Genes & Development, and BMC Cancer, among others. Research fields covered include DNA repair, replication fork protection, genome stability, phase separation, innate immune signaling, and cancer biology (prostate, breast, colorectal, glioma, leukemia, and lung cancer).
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Stalled replication fork protection limits cGAS-STING and P-body-dependent innate immune signalling.
| ||
Mol Cell Microhomology-mediated end joining acts directly on replication forks to repair single-ended double-strand breaks. | ||
Mol Cell Isoform-specific function of NSD3 in DNA replication stress confers resistance to PARP inhibitors in prostate cancer. | ||
Mol Cell Dynamic control of RNA-DNA hybrid formation orchestrates DNA2 activation at stalled forks by RNAPII and DDX39A
| ||
Mol Cell DDX39A resolves replication fork-associated RNA-DNA hybrids to balance fork protection and cleavage for genomic stability maintenance |
