Biotin-conjugated DNAJB12 Polyclonal antibody

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  IHC staining of human colon cancer using Biotin-16780

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using Biotin-16780 (DNAJB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using Biotin-16780

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using Biotin-16780 (DNAJB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

DNAJB12 Polyclonal Antibody for IHC

Cat No. Biotin-16780

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Synonyms

DJ10, DKFZp586B2023, DNAJB12

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Tested Applications

• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-16780 targets DNAJB12 in IHC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10193

  Product name: Recombinant human DNAJB12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 2-248 aa of BC011812

  Sequence: AYRRLALKFHPDKNHAPGATEAFKAIGTAYAVLSNPEKRKQYDQFGDDKSQAARHGHGHGDFHRGFEADISPEDLFNMFFGGGFPSSNVHVYSNGRMRYTYQQRQDRRDNQGDGGLGVFVQLMPILILILVSALSQLMVSSPPYSLSPRPSVGHIHRRVTDHLGVVYYVGDTFSEEYTGSSLKTVERNVEDDYIANLRNNCWKEKQQKEGLLYRARYFGDTDMYHRAQKMGTPSCSRLSEVQASLHG

• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 12
• Calculated Molecular Weight: 375 aa, 42 kDa
• Observed Molecular Weight: 40-42 kDa
• GenBank Accession Number: BC011812
• Gene Symbol: DNAJB12
• Gene ID (NCBI): 54788
• RRID: AB_2934329
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NXW2
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.