DNAJB4 Monoclonal antibody, PBS Only (Capture)

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• DNAJB4 Monoclonal antibody, PBS Only (Capture) (67966-1-PBS)

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  WB analysis using 67966-1-Ig (same clone as 67966-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 67966-1-Ig (same clone as 67966-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67966-1-Ig (DNAJB4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67966-1-Ig (same clone as 67966-1-PBS)

  WB result of DNAJB4 antibody (67966-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJB4 transfected HeLa cells. This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67966-1-Ig (same clone as 67966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 67966-1-Ig (same clone as 67966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67966-1-Ig (DNAJB4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67966-1-Ig (same clone as 67966-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using DNAJB4 antibody (67966-1-Ig, Clone: 1F3H7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67966-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51770-1

  Cytometric bead array sample test of MP51770-1, DNAJB4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67966-1-PBS. Detection antibody: 67966-2-PBS.

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  Cytometric bead array standard curve of MP51770-1

  Cytometric bead array standard curve of MP51770-1, DNAJB4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67966-1-PBS. Detection antibody: 67966-2-PBS. Standard:Ag3756. Range: 3.125-100 ng/mL.

• Featured Product
• KD/KO Validated

DNAJB4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67966-1-PBS

Clone No.1F3H7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rabbit

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DjB4, DNAJW, Heat shock protein 40 homolog, HLJ1, HSP40 homolog

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Product Information

67966-1-PBS targets DNAJB4 as part of a matched antibody pair:

MP51770-1: 67966-1-PBS capture and 67966-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3756

  Product name: Recombinant human DNAJB4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-337 aa of BC034721

  Sequence: MGKDYYCILGIEKGASDEDIKKAYRKQALKFHPDKNKSPQAEEKFKEVAEAYEVLSDPKKREIYDQFGEEGLKGGAGGTDGQGGTFRYTFHGDPHATFAAFFGGSNPFEIFFGRRMGGGRDSEEMEIDGDPFSAFGFSMNGYPRDRNSVGPSRLKQDPPVIHELRVSLEEIYSGCTKRMKISRKRLNADGRSYRSEDKILTIEIKKGWKEGTKITFPREGDETPNSIPADIVFIIKDKDHPKFKRDGSNIIYTAKISLREALCGCSINVPTLDGRNIPMSVNDIVKPGMRRRIIGYGLPFPKNPDQRGDLLIEFEVSFPDTISSSSKEVLRKHLPAS

• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 4
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC034721
• Gene Symbol: DNAJB4
• Gene ID (NCBI): 11080
• RRID: AB_2918717
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UDY4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.