DNAJB6 Monoclonal antibody, PBS Only

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  WB analysis of HEK-293 using 66587-1-Ig (same clone as 66587-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66587-1-Ig (same clone as 66587-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 66587-1-Ig (same clone as 66587-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66587-1-Ig (same clone as 66587-1-PBS)

  WB result of DNAJB6 antibody (66587-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJB6 transfected HeLa cells. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66587-1-Ig (same clone as 66587-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

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  WB analysis of Neuro-2a using 66587-1-Ig (same clone as 66587-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66587-1-Ig (same clone as 66587-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66587-1-Ig (same clone as 66587-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66587-1-Ig (same clone as 66587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 66587-1-Ig (same clone as 66587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66587-1-Ig (same clone as 66587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66587-1-Ig (same clone as 66587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66587-1-Ig (same clone as 66587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66587-1-Ig (same clone as 66587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66587-1-Ig (same clone as 66587-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNAJB6 antibody (66587-1-Ig, Clone: 1A1D2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66587-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

DNAJB6 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 66587-1-PBS

Clone No.1A1D2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

DJ4, DnaJ, Heat shock protein J2, HHDJ1, HSJ 2

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Product Information

66587-1-PBS targets DNAJB6 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2258

  Product name: Recombinant human DNAJB6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-241 aa of BC000177

  Sequence: MVDYYEVLGVQRHASPEDIKKAYRKLALKWHPDKNPENKEEAERKFKQVAEAYEVLSDAKKRDIYDKYGKEGLNGGGGGGSHFDSPFEFGFTFRNPDDVFREFFGGRDPFSFDFFEDPFEDFFGNRRGPRGSRSRGTGSFFSAFSGFPSFGSGFSSFDTGFTSFGSLGHGGLTSFSSTSFGGSGMGNFKSISTSTKMVNGRKITTKRIVENGQERVEVEEDGQLKSLTINGKEQLLRLDNK

• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 6
• Calculated Molecular Weight: 241 aa, 27 kDa
• Observed Molecular Weight: 27 kDa, 38 kDa
• GenBank Accession Number: BC000177
• Gene Symbol: DNAJB6
• Gene ID (NCBI): 10049
• RRID: AB_2881947
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O75190
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID: 20159555). The antibody 66587-1-Ig recognizes both isoforms.