DNAJB6 Monoclonal antibody

• Featured Product
• KD/KO Validated

DNAJB6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1A1D2

Cat no : 66587-1-Ig

Synonyms

DJ4, DnaJ, DNAJB6, Heat shock protein J2, HHDJ1, HSJ 2, HSJ2, MRJ, MSJ 1, MSJ1

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Validation Data Gallery

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  WB analysis of HEK-293 using 66587-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66587-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 66587-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66587-1-Ig

  WB result of DNAJB6 antibody (66587-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJB6 transfected HeLa cells.

• 

  WB analysis of pig brain using 66587-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66587-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66587-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 66587-1-Ig

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66587-1-Ig (DNAJB6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 66587-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 66587-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66587-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66587-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66587-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66587-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66587-1-Ig (DNAJB6 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, HSC-T6 cells, Neuro-2a cells, pig brain tissue, rat brain tissue, mouse brain tissue, HeLa cells
• Positive IHC detected in: human testis tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

66587-1-Ig targets DNAJB6 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DNAJB6 fusion protein Ag2258
• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 6
• Calculated Molecular Weight: 241 aa, 27 kDa
• Observed Molecular Weight: 27 kDa, 38 kDa
• GenBank Accession Number: BC000177
• Gene Symbol: DNAJB6
• Gene ID (NCBI): 10049
• RRID: AB_2881947
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJB6, belongs to the heat shock protein 40 (HSP40) family, a noncanonical member of the DNAJ-chaperone family, is mainly expressed in neurons. It plays an important role in recovery from misfolded protein aggregates (PMID: 27276715). As seen by the western blot stained for DNAJB6, there are 2 splice forms of the protein resulting in a longer isoform (38 kDa) and a shorter one (27 kDa). DNAJB6 (27 kDa) isoform but not DNAJB6 (38 kDa) isoform is effective with suppressing cytoplasmic protein aggregation (PMID: 20159555). The antibody 66587-1-Ig recognizes both isoforms.

Publications

Species	Application	Title
human	IHC	Transl Oncol; Development of novel DNAJB6-KIAA1522-p-mTOR three-protein prognostic prediction models for CRC; Authors - Yu-Juan Jiang
human	WB	Int J Biol Macromol; DnaJs are enriched in tau regulators; Authors - Abigail R Esquivel ; KD Validated
human	IF	Glia; DNAJB6 is expressed in neurons and oligodendrocytes of the human brain; Authors - Jónvá Hentze
human	WB,IHC	Biomedicines; Abnormally High Expression of DNAJB6 Accelerates Malignant Progression of Lung Adenocarcinoma; Authors - Di Wang ; KD Validated