Tested Applications
| Positive WB detected in | mouse kidney tissue, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13157-1-AP targets DNAJB9 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3789 Product name: Recombinant human DNAJB9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-223 aa of BC028912 Sequence: SYYDILGVPKSASERQIKKAFHKLAMKYHPDKNKSPDAEAKFREIAEAYETLSDANRRKEYDTLGHSAFTSGKGQRGSGSSFEQSFNFNFDDLFKDFGFFGQNQNTGSKKRFENHFQTRQDGGSSRQRHHFQEFSFGGGLFDDMFEDMEKMFSFSGFDSTNQHTVQTENRFHGSSKHCRTVTQRRGNMVTTYTDCSGQ Predict reactive species |
| Full Name | DnaJ (Hsp40) homolog, subfamily B, member 9 |
| Calculated Molecular Weight | 223 aa, 26 kDa |
| Observed Molecular Weight | 26-30 kDa |
| GenBank Accession Number | BC028912 |
| Gene Symbol | DNAJB9 |
| Gene ID (NCBI) | 4189 |
| RRID | AB_2877918 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBS3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNAJB9 is a cochaperone of heat shock protein 70 and is involved in endoplasmic reticulum protein-folding pathways.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DNAJB9 antibody 13157-1-AP | Download protocol |
| IP protocol for DNAJB9 antibody 13157-1-AP | Download protocol |
| WB protocol for DNAJB9 antibody 13157-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-DNAJB9 antibody (Cat# 13157-1-AP) has 9 total citations published in journals including Molecular Cell, Cell Death & Disease, Developmental Cell, Journal of Pineal Research, iScience, Human Molecular Genetics, Pathology International, eLife, and Case Reports in Nephrology. Research fields span ER stress and unfolded protein response, triple-negative breast cancer metastasis, pancreatic oncogenesis, glucose and lipid metabolism, epithelial-mesenchymal transition, cancer cell mitosis, and fibrillary glomerulonephritis.
| Species | Application | Title |
|---|---|---|
Mol Cell Coordination between Two Branches of the Unfolded Protein Response Determines Apoptotic Cell Fate. | ||
Cell Death Dis DNAJB9 suppresses the metastasis of triple-negative breast cancer by promoting FBXO45-mediated degradation of ZEB1.
| ||
Dev Cell ER-phagy and proteostasis defects prime pancreatic epithelial state changes in KRAS-mediated oncogenesis. | ||
J Pineal Res Melatonin alleviates PM2.5 -induced glucose metabolism disorder and lipidome alteration by regulating endoplasmic reticulum stress | ||
iScience DNAJB9 promotes epithelial-mesenchymal transition in the nasal epithelium of chronic rhinosinusitis by enhancing TRIM22-mediated IκBα degradation. | ||
Hum Mol Genet XBP1 variant 1 (Xv1) promotes mitosis of cancer cells involving upregulation of the polyglutamylase TTLL6. |
