DNAJC5 Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• DNAJC5 Polyclonal antibody, PBS Only (32204-1-PBS)

• 

  WB analysis using 32204-1-AP (same clone as 32204-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32204-1-AP (DNAJC5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 32204-1-AP (same clone as 32204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 32204-1-AP (same clone as 32204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 32204-1-AP (same clone as 32204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 32204-1-AP (same clone as 32204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 32204-1-AP (same clone as 32204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 32204-1-AP (same clone as 32204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 32204-1-AP (same clone as 32204-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNAJC5 antibody (32204-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

DNAJC5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 32204-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Ceroid-lipofuscinosis neuronal protein 4, CLN4, CSP, Cysteine string protein, DnaJ (Hsp40) Homolog, Subfamily C, Member 5

---

Product Information

32204-1-PBS targets DNAJC5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag35443

  Product name: Recombinant human DNAJC5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 110-198 aa of NM_025219.2

  Sequence: FVFCGLLTCCYCCCCLCCCFNCCCGKCKPKAPEGEETEFYVSPEDLEAQLQSDEREATDTPIVIQPASATETTQLTADSHPSYHTDGFN

• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 5
• Calculated Molecular Weight: 22kDa，198aa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: NM_025219.2
• Gene Symbol: DNAJC5
• Gene ID (NCBI): 80331
• RRID: AB_3670222
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9H3Z4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DNAJC5 (DnaJ homolog subfamily C member 5), also known as cysteine string protein α (CSPα), is a member of the HSP40 (DNAJ) family of proteins, as well as a co-chaperone of HSC70, and has been shown to control the extracellular release of many neurodegenerative disease proteins. DNAJC5 contains three structural domains - -a common N-terminal J structural domain conserved in DNAJ proteins, the cysteine string (CS) central structural domain, which is highly palmitoylated and anchors the protein to late endosomes, and an overall disordered C-terminal structural domain. Mutations in DNAJC5 have been strongly implicated in the development of human diseases such as neuronal waxy lipofuscinosis, cystic fibrosis, Huntington's disease and Parkinson's disease.