DNAJC7 Polyclonal antibody

• Featured Product
• KD/KO Validated

DNAJC7 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11090-1-AP

Synonyms

DANJC7, DJ11, DNAJC7, TPR repeat protein 2, TPR2, TTC2

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Validation Data Gallery

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  WB analysis using 11090-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11090-1-AP (DNAJC7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11090-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11090-1-AP (DNAJC7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11090-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11090-1-AP (DNAJC7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11090-1-AP

  Immunofluorescent analysis of HepG2 cells, using DNAJC7 antibody 11090-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 11090-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug DNAJC7 antibody (11090-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, Jurkat cells, moust testis
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

11090-1-AP targets DNAJC7 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJC7 fusion protein Ag1572
• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 7
• Calculated molecular weight: 56 kDa
• Observed molecular weight: 56 kDa
• GenBank accession number: BC011837
• Gene symbol: DNAJC7
• Gene ID (NCBI): 7266
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	CoIP	Nat Commun; DnaJC7 binds natively folded structural elements in tau to inhibit amyloid formation.; Authors - Zhiqiang Hou
human	WB,IF	Biochem Pharmacol; Indirect activation of constitutive androstane receptor in three-dimensionally cultured HepG2 cells.; Authors - Kosuke Yokobori ; KO Validated
human	WB	Biochem Biophys Res Commun; Co-chaperon DnaJC7/TPR2 enhances p53 stability and activity through blocking the complex formation between p53 and MDM2.; Authors - Kubo Natsumi N
human	WB	bioRxiv; DnaJC7 specifically regulates tau seeding; Authors - Valerie A Perez ; KO Validated
human	WB	Cancers (Basel); Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin; Authors - Mohamed Alalem
human	WB	Life Sci Alliance; ALS-linked loss of Cyclin-F function affects HSP90; Authors - Alexander Siebert