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  • KD/KO Validated

DNAJC7 Polyclonal antibody

DNAJC7 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11090-1-AP

Synonyms

DANJC7, DJ11, DNAJC7, TPR repeat protein 2, TPR2, TTC2



Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, Jurkat cells, moust testis
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11090-1-AP targets DNAJC7 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNAJC7 fusion protein Ag1572
Full Name DnaJ (Hsp40) homolog, subfamily C, member 7
Calculated molecular weight 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC011837
Gene symbol DNAJC7
Gene ID (NCBI) 7266
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DNAJC7 antibody 11090-1-APDownload protocol
IHC protocol for DNAJC7 antibody 11090-1-APDownload protocol
IF protocol for DNAJC7 antibody 11090-1-APDownload protocol
FC protocol for DNAJC7 antibody 11090-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseCoIP

Nat Commun

DnaJC7 binds natively folded structural elements in tau to inhibit amyloid formation.

Authors - Zhiqiang Hou
humanWB,IF

Biochem Pharmacol

Indirect activation of constitutive androstane receptor in three-dimensionally cultured HepG2 cells.

Authors - Kosuke Yokobori
  • KO Validated
humanWB

Biochem Biophys Res Commun

Co-chaperon DnaJC7/TPR2 enhances p53 stability and activity through blocking the complex formation between p53 and MDM2.

Authors - Kubo Natsumi N
humanWB

bioRxiv

DnaJC7 specifically regulates tau seeding

Authors - Valerie A Perez
  • KO Validated
humanWB

Cancers (Basel)

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin

Authors - Mohamed Alalem
humanWB

Life Sci Alliance

ALS-linked loss of Cyclin-F function affects HSP90

Authors - Alexander Siebert