DNALI1 Recombinant antibody, PBS Only

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  WB analysis using 85734-1-RR (same clone as 85734-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85734-1-RR (DNALI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85734-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse fallopian tube using 85734-1-RR (same clone as 85734-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue slide using 85734-1-RR (DNALI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85734-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85734-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85734-1-RR against Human DNALI1 were performed. The affinity constant is below 1 pM.

DNALI1 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 85734-1-PBS

Clone No.243157E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

hp28, P28

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Product Information

85734-1-PBS targets DNALI1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DNALI1 fusion protein Ag11652
• Full Name: dynein, axonemal, light intermediate chain 1
• Calculated Molecular Weight: 258 aa, 30 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC046117
• Gene Symbol: DNALI1
• Gene ID (NCBI): 7802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14645
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DNALI1 belongs to the inner dynein arm light chain family and encodes a subunit of the light intermediate chain of inner dynein arms of the axoneme of sperm flagella (PMID: 36792588). DNALI1 promotes neurodegeneration after traumatic brain injury via inhibition of autophagosome-lysosome fusion (PMID: 38348540). Mutation in DNALI1 Causes Male Infertility with Severe Oligoasthenoteratozoospermia in Humans (PMID: 38212584).