DNALI1 Recombinant antibody

DNALI1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85734-1-RR
Clone No.243157E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

hp28, P28

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse fallopian tube tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85734-1-RR targets DNALI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DNALI1 fusion protein Ag11652 Predict reactive species
Full Name dynein, axonemal, light intermediate chain 1
Calculated Molecular Weight 258 aa, 30 kDa
Observed Molecular Weight 30-33 kDa
GenBank Accession NumberBC046117
Gene Symbol DNALI1
Gene ID (NCBI) 7802
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14645
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNALI1 belongs to the inner dynein arm light chain family and encodes a subunit of the light intermediate chain of inner dynein arms of the axoneme of sperm flagella (PMID: 36792588). DNALI1 promotes neurodegeneration after traumatic brain injury via inhibition of autophagosome-lysosome fusion (PMID: 38348540). Mutation in DNALI1 Causes Male Infertility with Severe Oligoasthenoteratozoospermia in Humans (PMID: 38212584).

Protocols

Product Specific Protocols
WB protocol for DNALI1 antibody 85734-1-RRDownload protocol
IHC protocol for DNALI1 antibody 85734-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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