DNALI1 Recombinant monoclonal antibody

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  WB analysis using 85734-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85734-1-RR (DNALI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse fallopian tube using 85734-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue slide using 85734-1-RR (DNALI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85734-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85734-1-RR against Human DNALI1 were performed. The affinity constant is below 1 pM.

DNALI1 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 85734-1-RR

Clone No.243157E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

hp28, P28

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IHC detected in: mouse fallopian tube tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85734-1-RR targets DNALI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag11652

  Product name: Recombinant human DNALI1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-258 aa of BC046117

  Sequence: MIPPADSLLKYDTPVLVSRNTEKRSPKARLLKVSPQQPGPSGSAPQPPKTKLPSTPCVPDPTKQAEEILNAILPPREWVEDTQLWIQQVSSTPSTRMDVVHLQEQLDLKLQQRQARETGICPVRRELYSQCFDELIREVTINCAERGLLLLRVRDEIRMTIAAYQTLYESSVAFGMRKALQAEQGKSDMERKIAELETEKRDLERQVNEQKAKCEATEKRESERRQVEEKKHNEEIQFLKRTNQQLKAQLEGIIAPKK

• Full Name: dynein, axonemal, light intermediate chain 1
• Calculated Molecular Weight: 258 aa, 30 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC046117
• Gene Symbol: DNALI1
• Gene ID (NCBI): 7802
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14645
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNALI1 belongs to the inner dynein arm light chain family and encodes a subunit of the light intermediate chain of inner dynein arms of the axoneme of sperm flagella (PMID: 36792588). DNALI1 promotes neurodegeneration after traumatic brain injury via inhibition of autophagosome-lysosome fusion (PMID: 38348540). Mutation in DNALI1 Causes Male Infertility with Severe Oligoasthenoteratozoospermia in Humans (PMID: 38212584).