Tested Applications
Positive WB detected in | mouse testis tissue, rat testis tissue |
Positive IHC detected in | mouse fallopian tube tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
85734-1-RR targets DNALI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | DNALI1 fusion protein Ag11652 Predict reactive species |
Full Name | dynein, axonemal, light intermediate chain 1 |
Calculated Molecular Weight | 258 aa, 30 kDa |
Observed Molecular Weight | 30-33 kDa |
GenBank Accession Number | BC046117 |
Gene Symbol | DNALI1 |
Gene ID (NCBI) | 7802 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O14645 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNALI1 belongs to the inner dynein arm light chain family and encodes a subunit of the light intermediate chain of inner dynein arms of the axoneme of sperm flagella (PMID: 36792588). DNALI1 promotes neurodegeneration after traumatic brain injury via inhibition of autophagosome-lysosome fusion (PMID: 38348540). Mutation in DNALI1 Causes Male Infertility with Severe Oligoasthenoteratozoospermia in Humans (PMID: 38212584).
Protocols
Product Specific Protocols | |
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WB protocol for DNALI1 antibody 85734-1-RR | Download protocol |
IHC protocol for DNALI1 antibody 85734-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |