DNASE1L1 Polyclonal antibody

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  WB analysis of mouse skeletal muscle using 13653-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13653-1-AP (DNASE1L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 13653-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13653-1-AP (DNASE1L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 13653-1-AP

  IP result of anti-DNASE1L1 (IP:13653-1-AP, 4ug; Detection:13653-1-AP 1:800) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human skeletal muscle using 13653-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13653-1-AP (DNASE1L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 13653-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 13653-1-AP (DNASE1L1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 13653-1-AP

  Immunofluorescent analysis of MCF-7 cells, using DNASE1L1 antibody 13653-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• Featured Product
• KD/KO Validated

DNASE1L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13653-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

DNase I-like 1, DNase I like 1, DNAS1L1, Deoxyribonuclease-1-like 1, Deoxyribonuclease I-like 1

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

13653-1-AP targets DNASE1L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4222

  Product name: Recombinant human DNASE1L1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-302 aa of BC028092

  Sequence: MHYPTALLFLILANGAQAFRICAFNAQRLTLAKVAREQVMDTLVRILARCDIMVLQEVVDSSGSAIPLLLRELNRFDGSGPYSTLSSPQLGRSTYMETYVYFYRSHKTQVLSSYVYNDEDDVFAREPFVAQFSLPSNVLPSLVLVPLHTTPKAVEKELNALYDVFLEVSQHWQSKDVILLGDFNADCASLTKKRLDKLELRTEPGFHWVIADGEDTTVRASTHCTYDRVVLHGERCRSLLHTAAAFDFPTSFQLTEEEALNISDHYPVEVELKLSQAHSVQPLSLTVLLLLSLLSPQLCPAA

• Full Name: deoxyribonuclease I-like 1
• Calculated Molecular Weight: 302 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC028092
• Gene Symbol: DNASE1L1
• Gene ID (NCBI): 1774
• RRID: AB_2230813
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49184
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IF	Cells; New Findings: Hindlimb Unloading Causes Nucleocytoplasmic Ca2+ Overload and DNA Damage in Skeletal Muscle; Authors - Huajian Yang
rat	WB	J Tradit Chin Med; Synergistic effect of schizandrin A and DNase I knockdown on high glucose induced beta cell apoptosis by decreasing intracellular calcium concentration; Authors - Zhu Bin ; KD Validated