DNASE1L1 Polyclonal antibody

DNASE1L1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13653-1-AP

Synonyms

Deoxyribonuclease 1 like 1, deoxyribonuclease I like 1, DNAS1L1, DNase I like 1, DNase X, DNASE1L1, DNASEX, DNL1L, Muscle specific DNase I like, XIB



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13653-1-AP targets DNASE1L1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNASE1L1 fusion protein Ag4222
Full Name deoxyribonuclease I-like 1
Calculated Molecular Weight 302 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC028092
Gene Symbol DNASE1L1
Gene ID (NCBI) 1774
RRIDAB_2230813
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DNASE1L1 antibody 13653-1-APDownload protocol
IHC protocol for DNASE1L1 antibody 13653-1-APDownload protocol
IF protocol for DNASE1L1 antibody 13653-1-APDownload protocol
IP protocol for DNASE1L1 antibody 13653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Cells

New Findings: Hindlimb Unloading Causes Nucleocytoplasmic Ca2+ Overload and DNA Damage in Skeletal Muscle

Authors - Huajian Yang
ratWB

J Tradit Chin Med

Synergistic effect of schizandrin A and DNase I knockdown on high glucose induced beta cell apoptosis by decreasing intracellular calcium concentration

Authors - Zhu Bin
  • KD Validated