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DOCK5 Polyclonal antibody, PBS Only (29938-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 29938-1-AP (same clone as 29938-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.
WB analysis using 29938-1-AP (same clone as 29938-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.
WB analysis using 29938-1-AP (same clone as 29938-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 29938-1-AP (DOCK5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 29938-1-AP (same clone as 29938-1-PBS)
WB result of DOCK5 antibody (29938-1-AP; 1:2400; incubated at room temperature for 1.5 hours) with sh-Control and sh-DOCK5 transfected A549 cells. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.
IP experiment of DU 145 using 29938-1-AP (same clone as 29938-1-PBS)
IP result of anti-DOCK5 (IP:29938-1-AP, 4ug; Detection:29938-1-AP 1:2000) with DU 145 cells lysate 1280 ug. This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 29938-1-AP (same clone as 29938-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29938-1-AP (DOCK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.
IHC staining of human intrahepatic cholangiocarcinoma using 29938-1-AP (same clone as 29938-1-PBS)
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29938-1-AP (DOCK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.
IHC staining of human intrahepatic cholangiocarcinoma using 29938-1-AP (same clone as 29938-1-PBS)
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29938-1-AP (DOCK5 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 29938-1-AP (same clone as 29938-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DOCK5 antibody (29938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29938-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

DOCK5 Polyclonal antibody, PBS Only

Name: DOCK5 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 29938-1-PBS

DOCK5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 29938-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

dedicator of cytokinesis 5

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

29938-1-PBS targets DOCK5 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag32193 Product name: Recombinant human DOCK5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1700-1870 aa of BC137175 Sequence: ILEPLLERRASSGARVEDLSLREENSENRISKFKRKDWSLSKSQVIAEKAPEPDLMSPTRKAQRPKSLQLMDNRLSPFHGSSPPQSTPLSPPPLTPKATRTLSSPSLQTDGIAATPVPPPPPPKSKPYEGSQRNSTELAPPLPVRREAKAPPPPPPKARKSGIPTSEPGSQ Predict reactive species
Full Name	dedicator of cytokinesis 5
Calculated Molecular Weight	1870 aa, 215 kDa
Observed Molecular Weight	190 kDa
GenBank Accession Number	BC137175
Gene Symbol	DOCK5
Gene ID (NCBI)	80005
RRID	AB_3086194
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H7D0
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

DOCK5 is a member of the dedicator of cytokinesis protein family. Members of this family act as guanine nucleotide exchange factors for small Rho family G proteins. DOCK5 is thought to associate with adaptors CRK and CRKL, and function in regulation of intestinal epithelial cell spreading and migration on collagen IV.