DPP10 Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA sample test of MP01035-3

  HT-29 cell lysate was measured. The human DPP10 concentration of detected samples was determined to be 3,448.25 ng/mL (based on a 4.4 mg/mL extract load).

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  Sandwich ELISA standard curve of MP01035-3

  Sandwich ELISA standard curve of MP01035-3, Human DPP10 Recombinant Matched Antibody Pair - PBS only. 84127-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3231. 84127-3-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 ng/mL

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  Affinity and Kinetic Characterization of 84127-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 84127-3-PBS against Human DPP10 was performed. The affinity constant is 0.803 nM.

DPP10 Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Cat No. 84127-3-PBS

Clone No.241446G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA

Synonyms

dipeptidyl peptidase 10, Dipeptidyl peptidase IV-related protein 3, Dipeptidyl peptidase X, Dipeptidyl peptidase-like protein 2, DPL2

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Product Information

84127-3-PBS targets DPP10 as part of a matched antibody pair:

MP01035-3: 84127-2-PBS capture and 84127-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3231

  Product name: Recombinant human DPP10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 448-796 aa of BC030832

  Sequence: RGRQLYSASTEGLLNRQCISCNFMKEQCTYFDASFSPMNQHFLLFCEGPRVPVVSLHSTDNPAKYFILESNSMLKEAILKKKIGKPEIKILHIDDYELPLQLSLPKDFMDRNQYALLLIMDEEPGGQLVTDKFHIDWDSVLIDMDNVIVARFDGRGSGFQGLKILQEIHRRLGSVEVKDQITAVKFLLKLPYIDSKRLSIFGKGYGGYIASMILKSDEKLFKCGSVVAPITDLKLYASAFSERYLGMPSKEESTYQAASVLHNVHGLKEENILIIHGTADTKVHFQHSAELIKHLIKAGVNYTMQVYPDEGHNVSEKSKYHLYSTILKFFSDCLKEEISVLPQEPEEDE

• Full Name: dipeptidyl-peptidase 10
• Calculated Molecular Weight: 796 aa, 91 kDa
• GenBank Accession Number: BC030832
• Gene Symbol: DPP10
• Gene ID (NCBI): 57628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N608
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.