DPP4/CD26 Polyclonal antibody
DPP4/CD26 Polyclonal Antibody for FC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (1)
Applications
WB, FC, ELISA
Conjugate
Unconjugated
Cat no : 29403-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Caco-2 cells, HT-29 cells |
Positive FC detected in | HT-29 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 1 publications below |
Product Information
29403-1-AP targets DPP4/CD26 in WB, FC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | DPP4/CD26 fusion protein Ag30160 |
Full Name | dipeptidyl-peptidase 4 |
Calculated molecular weight | 88 kDa |
Observed molecular weight | 110 kDa |
GenBank accession number | BC013329 |
Gene symbol | DPP4 |
Gene ID (NCBI) | 1803 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DPP4 (also known as CD26) is a serine exopeptidase that cleaves X-proline dipeptides from the N terminus of polypeptides. It is an intrinsic membrane glycoprotein anchored into the cell membrane by its N-terminal end. High levels of the enzyme are found in the brush-border membranes of the kidney proximal tubule and of the small intestine, but several other tissues also express the enzyme. The enzyme is present in the fetal colon but disappears at birth. It is ectopically expressed in some human colon adenocarcinomas and human colon cancer cell lines (PMID:1977364).
Protocols
Product Specific Protocols | |
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WB protocol for DPP4/CD26 antibody 29403-1-AP | Download protocol |
FC protocol for DPP4/CD26 antibody 29403-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Anal Chem Ultrasensitive Fluorescent Probes Reveal an Adverse Action of Dipeptide Peptidase IV and Fibroblast Activation Protein during Proliferation of Cancer Cells |