DPP4/CD26 Polyclonal antibody

DPP4/CD26 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 29403-1-AP

Synonyms

ADABP, ADCP 2, ADCP2, CD26, dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, DPP4, DPPIV, T cell activation antigen CD26, TP103

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Validation Data Gallery

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  WB analysis using 29403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29403-1-AP (DPP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of HT-29 using 29403-1-AP

  1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human DPP4/CD26 (29403-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: Caco-2 cells, HT-29 cells
• Positive FC detected in: HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

29403-1-AP targets DPP4/CD26 in WB, FC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DPP4/CD26 fusion protein Ag30160
• Full Name: dipeptidyl-peptidase 4
• Calculated molecular weight: 88 kDa
• Observed molecular weight: 110 kDa
• GenBank accession number: BC013329
• Gene symbol: DPP4
• Gene ID (NCBI): 1803
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DPP4 (also known as CD26) is a serine exopeptidase that cleaves X-proline dipeptides from the N terminus of polypeptides. It is an intrinsic membrane glycoprotein anchored into the cell membrane by its N-terminal end. High levels of the enzyme are found in the brush-border membranes of the kidney proximal tubule and of the small intestine, but several other tissues also express the enzyme. The enzyme is present in the fetal colon but disappears at birth. It is ectopically expressed in some human colon adenocarcinomas and human colon cancer cell lines (PMID:1977364).

Publications

Species	Application	Title
human	WB	Anal Chem; Ultrasensitive Fluorescent Probes Reveal an Adverse Action of Dipeptide Peptidase IV and Fibroblast Activation Protein during Proliferation of Cancer Cells; Authors - Qiuyu Gong