DPP4/CD26 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84606-6-RR (same clone as 84606-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84606-6-RR (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84606-6-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 84606-6-RR (same clone as 84606-6-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 84606-6-RR (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84606-6-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 84606-6-RR (same clone as 84606-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84606-6-RR (DPP4/CD26 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84606-6-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 84606-6-RR (same clone as 84606-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84606-6-RR (DPP4/CD26 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84606-6-PBS in a different storage buffer formulation.

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  IF Staining of HuH-7 using 84606-6-RR (same clone as 84606-6-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HuH-7 cells using DPP4/CD26 antibody (84606-6-RR, Clone: 242045F5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84606-6-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 84606-6-RR (same clone as 84606-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using DPP4/CD26 antibody (84606-6-RR, Clone: 242045F5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84606-6-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84606-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84606-6-RR against Human DPP4/CD26 were performed. The affinity constant is 1.59 nM.

DPP4/CD26 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84606-6-PBS

Clone No.242045F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CD26, 242045F5, ADABP, ADCP 2, ADCP2

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Product Information

84606-6-PBS targets DPP4/CD26 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0157

  Product name: Recombinant Human DPP4/CD26 protein (Myc Tag, His Tag)

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: Myc & 6*His

  Domain: 34-766 aa of BC013329

  Sequence: DATADSRKTYTLTDYLKNTYRLKLYSLRWISDHEYLYKQENNILVFNAEYGNSSVFLENSTFDEFGHSINDYSISPDGQFILLEYNYVKQWRHSYTASYDIYDLNKRQLITEERIPNNTQWVTWSPVGHKLAYVWNNDIYVKIEPNLPSYRITWTGKEDIIYNGITDWVYEEEVFSAYSALWWSPNGTFLAYAQFNDTEVPLIEYSFYSDESLQYPKTVRVPYPKAGAVNPTVKFFVVNTDSLSSVTNATSIQITAPASMLIGDHYLCDVTWATQERISLQWLRRIQNYSVMDICDYDESSGRWNCLVARQHIEMSTTGWVGRFRPSEPHFTLDGNSFYKIISNEEGYRHICYFQIDKKDCTFITKGTWEVIGIEALTSDYLYYISNEYKGMPGGRNLYKIQLSDYTKVTCLSCELNPERCQYYSVSFSKEAKYYQLRCSGPGLPLYTLHSSVNDKGLRVLEDNSALDKMLQNVQMPSKKLDFIILNETKFWYQMILPPHFDKSKKYPLLLDVYAGPCSQKADTVFRLNWATYLASTENIIVASFDGRGSGYQGDKIMHAINRRLGTFEVEDQIEAARQFSKMGFVDNKRIAIWGWSYGGYVTSMVLGSGSGVFKCGIAVAPVSRWEYYDSVYTERYMGLPTPEDNLDHYRNSTVMSRAENFKQVEYLLIHGTADDNVHFQQSAQISKALVDVGVDFQAMWYTDEDHGIASSTAHQHIYTHMSHFIKQCFSLP

• Full Name: dipeptidyl-peptidase 4
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC013329
• Gene Symbol: DPP4
• Gene ID (NCBI): 1803
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P27487
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).