CRMP2 Polyclonal antibody

CRMP2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC

Conjugate

Unconjugated

Publications

1

Cat no : 14521-1-AP

Synonyms

CRMP 2, CRMP2, DHPRP2, dihydropyrimidinase like 2, DPYSL2, DRP 2, DRP2, N2A3, ULIP 2, ULIP2, Unc 33 like phosphoprotein 2

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Validation Data Gallery

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  WB analysis using 14521-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14521-1-AP (CRMP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14521-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14521-1-AP (CRMP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 14521-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14521-1-AP (CRMP2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14521-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14521-1-AP (CRMP2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human heart tissue, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14521-1-AP targets CRMP2 in WB, IHC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRMP2 fusion protein Ag5987
• Full Name: dihydropyrimidinase-like 2
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC067109
• Gene Symbol: DPYSL2
• Gene ID (NCBI): 1808
• RRID: AB_2094341
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dihydropyrimidinase-like 2 (Dpysl2), a protein involved in neuronal differentiation and axonal guidance is found altered in various nervous system diseases (PMID:19524114). It is also named as CRMP2, ULIP2 and belongs to hydantoinase/dihydropyrimidinase subfamily. The phosphorylation of the particular portion carboxyl-terminal to the basic region of DPYSL2 may play an important role in regulating its activity, and may be involved in the formation of degenerating neurites in AD brain(PMID:10757975).

Publications

Species	Application	Title
human	WB	Neural Regen Res; GIT1 enhances neurite outgrowth by stimulating microtubule assembly.; Authors - Yi-Sheng Li