CRMP2 Polyclonal antibody

CRMP2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14686-1-AP

Synonyms

CRMP 2, CRMP2, DHPRP2, dihydropyrimidinase like 2, DPYSL2, DRP 2, DRP2, N2A3, ULIP 2, ULIP2, Unc 33 like phosphoprotein 2



Tested Applications

Positive WB detected inPC-12 cells, mouse brain tissue, rat brain tissue, SH-SY5Y cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14686-1-AP targets CRMP2 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRMP2 fusion protein Ag5985
Full Name dihydropyrimidinase-like 2
Calculated molecular weight 62 kDa
Observed molecular weight 62-66 kDa
GenBank accession numberBC056408
Gene symbol DPYSL2
Gene ID (NCBI) 1808
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dihydropyrimidinase-like 2 (Dpysl2), a protein involved in neuronal differentiation and axonal guidance is found altered in various nervous system diseases (PMID:19524114). It is also named as CRMP2, ULIP2 and belongs to hydantoinase/dihydropyrimidinase subfamily. The phosphorylation of the particular portion carboxyl-terminal to the basic region of DPYSL2 may play an important role in regulating its activity, and may be involved in the formation of degenerating neurites in AD brain(PMID:10757975).

Protocols

Product Specific Protocols
WB protocol for CRMP2 antibody 14686-1-APDownload protocol
IHC protocol for CRMP2 antibody 14686-1-APDownload protocol
IF protocol for CRMP2 antibody 14686-1-APDownload protocol
Standard Protocols
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