Tested Applications
| Positive WB detected in | PC-12 cells, mouse brain tissue, rat brain tissue, SH-SY5Y cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14686-1-AP targets CRMP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5985 Product name: Recombinant human DPYSL2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 223-572 aa of BC056408 Sequence: HVLSRPEEVEAEAVNRAITIANQTNCPLYITKVMSKSSAEVIAQARKKGTVVYGEPITASLGTDGSHYWSKNWAKAAAFVTSPPLSPDPTTPDFLNSLLSCGDLQVTGSAHCTFNTAQKAVGKDNFTLIPEGTNGTEERMSVIWDKAVVTGKMDENQFVAVTSTNAAKVFNLYPRKGRIAVGSDADLVIWDPDSVKTISAKTHNSSLEYNIFEGMECRGSPLVVISQGKIVLEDGTLHVTEGSGRYIPRKPFPDFVYKRIKARSRLAELRGVPRGLYDGPVCEVSVTPKTVTPASSAKTSPAKQQAPPVRNLHQSGFSLSGAQIDDNIPRRTTQRIVAPPGGRANITSLG Predict reactive species |
| Full Name | dihydropyrimidinase-like 2 |
| Calculated Molecular Weight | 62 kDa |
| Observed Molecular Weight | 62-66 kDa |
| GenBank Accession Number | BC056408 |
| Gene Symbol | CRMP2 |
| Gene ID (NCBI) | 1808 |
| RRID | AB_10638925 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16555 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Dihydropyrimidinase-like 2 (Dpysl2), a protein involved in neuronal differentiation and axonal guidance is found altered in various nervous system diseases (PMID:19524114). It is also named as CRMP2, ULIP2 and belongs to hydantoinase/dihydropyrimidinase subfamily. The phosphorylation of the particular portion carboxyl-terminal to the basic region of DPYSL2 may play an important role in regulating its activity, and may be involved in the formation of degenerating neurites in AD brain(PMID:10757975).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CRMP2 antibody 14686-1-AP | Download protocol |
| IHC protocol for CRMP2 antibody 14686-1-AP | Download protocol |
| WB protocol for CRMP2 antibody 14686-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CRMP2 Polyclonal antibody (catalog no. 14686-1-AP) has 8 citations. They appear in Cancer Research, Nature Communications, Neural Regeneration Research, CNS Neuroscience & Therapeutics, Biochimica et Biophysica Acta – Molecular Basis of Disease, European Journal of Medical Research, Bioactive Materials, and Respiratory Research. Research fields span cancer (centrosome amplification, lung adenocarcinoma), neuroscience (neurogenesis, stroke, spinal cord injury, anxiety, encephalopathy), and intervertebral disc degeneration.
| Species | Application | Title |
|---|---|---|
Cancer Res Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification. | ||
Nat Commun A branching model of lineage differentiation underpinning the neurogenic potential of enteric glia | ||
Neural Regen Res Adeno-associated virus-mediated inhibition of ROCK2 promotes synaptogenesis and neurogenesis in rats after ischemic stroke. | ||
CNS Neurosci Ther Autoantibodies Against Collapsin Response Mediator Proteins Associated With Encephalopathy/Myelopathy: A Single-Center Retrospective Study. | ||
Biochim Biophys Acta Mol Basis Dis Anti-CRMP2 antibody induces anxiety-like behavior and increases pyramidal neuron excitability in mice | ||
Eur J Med Res Screening and identification of differentially expressed miRNA and mRNA for intervertebral disc degeneration on bioinformatics. |
