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  • KD/KO Validated

DRD5 Polyclonal antibody

DRD5 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 20310-1-AP

Synonyms

dopamine receptor D5, Dopamine D5 receptor, DBDR, D1beta dopamine receptor, D(5) dopamine receptor



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20310-1-AP targets DRD5 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DRD5 fusion protein Ag14145
Full Name dopamine receptor D5
Calculated Molecular Weight 477 aa, 53 kDa
Observed Molecular Weight 50-53 kDa
GenBank Accession NumberBC009748
Gene Symbol DRD5
Gene ID (NCBI) 1816
RRIDAB_10699880
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DRD5, is a G-protein coupled receptor and involved in diversified physiological and pathological processes. this receptor stimulates adenylyl cyclase activity and is expressed in the limbic area of the mammalian brain(PMID:14699430). Studies have shown that DRD5 is highly expressed in colonic macrophages and DRD5 deficiency exacerbates DSS(dextran sodium sulfate)-induced colitis(PMID:34001860). in addition, DRD5 agonists were potent inhibitors of pituitary tumor growth(PMID:28613975).

Protocols

Product Specific Protocols
WB protocol for DRD5 antibody 20310-1-APDownload protocol
IHC protocol for DRD5 antibody 20310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

PMID: 25594175

Authors - Yiqing Yan
  • KD Validated
mouseWB

Mol Cell

Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation.

Authors - Yuqing Wu
  • KO Validated
mouseIHC

Int J Mol Sci

Gastrointestinal Dopamine in Inflammatory Bowel Diseases: A Systematic Review.

Authors - Magdalena Kurnik-Łucka
humanWB

ACS Chem Neurosci

Development of a PC12 Cell Based Assay for Screening Catechol-O-methyltransferase Inhibitors

Authors - Gongliang Zhang
humanWB,IF

Cell Oncol (Dordr)

Activation of dopamine receptor D1 inhibits glioblastoma tumorigenicity by regulating autophagic activity.

Authors - Kang Yang
mouseWB

J Neurochem

Validation of the Targeted Metabolomic Pathway in the Hippocampus and Comparative Analysis with the Prefrontal Cortex of Social Defeat Model Mice.

Authors - Ke Xu