Tested Applications
| Positive WB detected in | HepG2 cells, mouse brain tissue, SMMC-7721 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14743-1-AP targets DRG2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6433 Product name: Recombinant human DRG2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-364 aa of BC000493 Sequence: MGILEKISEIEKEIARTQKNKATEYHLGLLKAKLAKYRAQLLEPSKSASSKGEGFDVMKSGDARVALIGFPSVGKSTFLSLMTSTASEAASYEFTTLTCIPGVIEYKGANIQLLDLPGIIEGAAQGKGRGRQVIAVARTADVIIMMLDATKGEVQRSLLEKELESVGIRLNKHKPNIYFKPKKGGGISFNSTVMLTQCSEKLVQLILHEYKIFNAEVLFREDCSPDEFIDVIVGNRVYMPCLYVYNKIDQISMEEVDRLARKPNSVVISCGMKLNLDYLLEMLWEYLALTCIYTKKRGQRPDFTDAIILRKGASVEHVCHRIHRSLASQFKYALVWGTSTKYSPQRVGLTHTMEHEDVIQIVKK Predict reactive species |
| Full Name | developmentally regulated GTP binding protein 2 |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 41-45 kDa |
| GenBank Accession Number | BC000493 |
| Gene Symbol | DRG2 |
| Gene ID (NCBI) | 1819 |
| RRID | AB_2230800 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55039 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Developmentally regulated GTP-binding protein (DRG) is a new subfamily within the superfamily of GTP-binding proteins. Its expression is regulated during embryonic development. DRG2 plays a critical role in control of the cell cycle and apoptosis (PMID:27669826, PMID:14759600). DRG2 Regulates G2/M Progression via the Cyclin B1-Cdk1 Complex.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DRG2 antibody 14743-1-AP | Download protocol |
| IHC protocol for DRG2 antibody 14743-1-AP | Download protocol |
| IP protocol for DRG2 antibody 14743-1-AP | Download protocol |
| WB protocol for DRG2 antibody 14743-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
DRG2 Affinity Purification Antibody (Cat# 14743-1-AP) has 16 total citations across 14 journals, including Nature Chemical Biology, Nucleic Acids Research, Cell Death Discovery, Structure, FASEB Journal, and Clinical Immunology. Research fields span epigenetics, telomere biology, cancer (prostate, ATRX-null), cellular senescence, vascular biology, osteoclast differentiation, mitochondrial function, innate immunity, androgen signaling, and inflammatory pain.
| Species | Application | Title |
|---|---|---|
Nat Chem Biol The Jumonji-C oxygenase JMJD7 catalyzes (3S)-lysyl hydroxylation of TRAFAC GTPases. | ||
Nucleic Acids Res Elevated reactive oxygen species can drive the alternative lengthening of telomeres pathway in ATRX-null cancers | ||
Nucleic Acids Res Elevated reactive oxygen species can drive the alternative lengthening of telomeres pathway in ATRX-null cancers
| ||
Cell Death Discov DRG2 is required for surface localization of PD-L1 and the efficacy of anti-PD-1 therapy | ||
Mol Cells DRG2 Deficiency Causes Impaired Microtubule Dynamics in HeLa Cells.
| ||
Mol Ther Nucleic Acids Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes. |
Reviews
What customers say
One reviewer (5/5 stars) used this antibody at a 1:2000 dilution for Western Blot on leukemic T-cells. They reported a single clean band at ~40 kDa, consistent with the expected DRG2 molecular weight, with minimal background and no non-specific bands observed. Overall, the reviewer was highly satisfied with the specificity and signal quality of the product.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wojciech (Verified Customer) (09-12-2025) | The antibody against DRG2 protein showed a single band at ~40 kDa in T-cells, consistent with the expected molecular weight. The signal was clean with minimal background. No non-specific bands were observed.
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