DSG2 Recombinant monoclonal antibody, PBS Only

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• DSG2 Recombinant monoclonal antibody, PBS Only (87426-1-PBS)

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  WB analysis using 87426-1-RR (same clone as 87426-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87426-1-RR (DSG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87426-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 87426-1-RR (same clone as 87426-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DSG2 antibody (87426-1-RR, Clone: 251405G8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87426-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 87426-1-RR (same clone as 87426-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using DSG2 antibody (87426-1-RR, Clone: 251405G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 87426-1-PBS in a different storage buffer formulation.

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  Affinity of 87426-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87426-1-RR against Human DSG2 were performed. The affinity constant is 2.32 nM.

DSG2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 87426-1-PBS

Clone No.251405G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ARVC10, ARVD10, Cadherin family member 5, CDHF5, Desmoglein 2

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Product Information

87426-1-PBS targets DSG2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6509

  Product name: Recombinant human DSG2 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 50-609 aa of NM_001943.4

  Sequence: AWITAPVALREGEDLSKKNPIAKIHSDLAEERGLKITYKYTGKGITEPPFGIFVFNKDTGELNVTSILDREETPFFLLTGYALDARGNNVEKPLELRIKVLDINDNEPVFTQDVFVGSVEELSAAHTLVMKINATDADEPNTLNSKISYRIVSLEPAYPPVFYLNKDTGEIYTTSVTLDREEHSSYTLTVEARDGNGEVTDKPVKQAQVQIRILDVNDNIPVVENKVLEGMVEENQVNVEVTRIKVFDADEIGSDNWLANFTFASGNEGGYFHIETDAQTNEGIVTLIKEVDYEEMKNLDFSVIVANKAAFHKSIRSKYKPTPIPIKVKVKNVKEGIHFKSSVISIYVSESMDRSSKGQIIGNFQAFDEDTGLPAHARYVKLEDRDNWISVDSVTSEIKLAKLPDFESRYVQNGTYTVKIVAISEDYPRKTITGTVLINVEDINDNCPTLIEPVQTICHDAEYVNVTAEDLDGHPNSGPFSFSVIDKPPGMAEKWKIARQESTSVLLQQSEKKLGRSEIQFLISDNQGFSCPEKQVLTLTVCECLHGSGCREAQHDSYVG

• Full Name: desmoglein 2
• Calculated Molecular Weight: 122 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: NM_001943.4
• Gene Symbol: DSG2
• Gene ID (NCBI): 1829
• RRID: AB_3745538
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14126
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).