DSG2 Recombinant monoclonal antibody

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• DSG2 Recombinant monoclonal antibody (87426-1-RR)

• 

  WB analysis using 87426-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87426-1-RR (DSG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 87426-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DSG2 antibody (87426-1-RR, Clone: 251405G8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of MCF-7 using 87426-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using DSG2 antibody (87426-1-RR, Clone: 251405G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity of 87426-1-RR

  Biolayer interferometry (BLl) kinetic assays of 87426-1-RR against Human DSG2 were performed. The affinity constant is 2.32 nM.

DSG2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87426-1-RR

Clone No.251405G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ARVC10, ARVD10, Cadherin family member 5, CDHF5, Desmoglein 2

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, A431 cells
• Positive IF/ICC detected in: A431 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87426-1-RR targets DSG2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg6509

  Product name: Recombinant human DSG2 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 50-609 aa of NM_001943.4

  Sequence: AWITAPVALREGEDLSKKNPIAKIHSDLAEERGLKITYKYTGKGITEPPFGIFVFNKDTGELNVTSILDREETPFFLLTGYALDARGNNVEKPLELRIKVLDINDNEPVFTQDVFVGSVEELSAAHTLVMKINATDADEPNTLNSKISYRIVSLEPAYPPVFYLNKDTGEIYTTSVTLDREEHSSYTLTVEARDGNGEVTDKPVKQAQVQIRILDVNDNIPVVENKVLEGMVEENQVNVEVTRIKVFDADEIGSDNWLANFTFASGNEGGYFHIETDAQTNEGIVTLIKEVDYEEMKNLDFSVIVANKAAFHKSIRSKYKPTPIPIKVKVKNVKEGIHFKSSVISIYVSESMDRSSKGQIIGNFQAFDEDTGLPAHARYVKLEDRDNWISVDSVTSEIKLAKLPDFESRYVQNGTYTVKIVAISEDYPRKTITGTVLINVEDINDNCPTLIEPVQTICHDAEYVNVTAEDLDGHPNSGPFSFSVIDKPPGMAEKWKIARQESTSVLLQQSEKKLGRSEIQFLISDNQGFSCPEKQVLTLTVCECLHGSGCREAQHDSYVG

• Full Name: desmoglein 2
• Calculated Molecular Weight: 122 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: NM_001943.4
• Gene Symbol: DSG2
• Gene ID (NCBI): 1829
• RRID: AB_3745538
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14126
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).